Results 51 to 60 of about 5,141,166 (402)

Identification of proteins and miRNAs that specifically bind an mRNA in vivo

Nature Communications, 2019
The entire mRNA interactome capture has been established but identification of specific mRNA-binding proteins has been challenging. Here, the authors developed an in vivo RNA–protein crosslinking and RNA pulldown approach and characterized novel ...
Kathrin Theil, Koshi Imami, Nikolaus Rajewsky   +2 more
doaj   +1 more source

Multilevel evolution shapes the function of NB-LRR encoding genes in plant innate immunity

Frontiers in Plant Science, 2022
A sophisticated innate immune system based on diverse pathogen receptor genes (PRGs) evolved in the history of plant life. To reconstruct the direction and magnitude of evolutionary trajectories of a given gene family, it is critical to detect the ...
Maria Raffaella Ercolano, Daniela D’Esposito, Giuseppe Andolfo, Luigi Frusciante   +3 more
doaj   +1 more source

Parallel genetics of regulatory sequences using scalable genome editing in vivo

Cell Reports, 2021
Summary: How regulatory sequences control gene expression is fundamental for explaining phenotypes in health and disease. Regulatory elements must ultimately be understood within their genomic environment and development- or tissue-specific contexts ...
Jonathan J. Froehlich, Bora Uyar, Margareta Herzog, Kathrin Theil, Petar Glažar, Altuna Akalin, Nikolaus Rajewsky   +6 more
doaj  

3D Chromatin Architecture Re-Wiring at the CDH3/CDH1 Loci Contributes to E-Cadherin to P-Cadherin Expression Switch in Gastric Cancer

Biology, 2023
Cadherins are cell–cell adhesion molecules, fundamental for cell architecture and polarity. E-cadherin to P-cadherin switch can rescue adherens junctions in epithelial tumours.
Celina São José, Carla Pereira, Marta Ferreira, Ana André, Hugo Osório, Irene Gullo, Fátima Carneiro, Carla Oliveira   +7 more
doaj   +1 more source

Rapid nuclear deadenylation of mammalian messenger RNA

iScience, 2023
Summary: Poly(A) tails protect RNAs from degradation and their deadenylation rates determine RNA stability. Although poly(A) tails are generated in the nucleus, deadenylation of tails has mostly been investigated within the cytoplasm.
Jonathan Alles, Ivano Legnini, Maddalena Pacelli, Nikolaus Rajewsky   +3 more
doaj  

Role of Genetic Variation in Transcriptional Regulatory Elements in Heart Rhythm

Cells, 2023
Genetic predisposition to cardiac arrhythmias has been a field of intense investigation. Research initially focused on rare hereditary arrhythmias, but over the last two decades, the role of genetic variation (single nucleotide polymorphisms) in heart ...
Timo Jonker, Phil Barnett, Gerard J. J. Boink, Vincent M. Christoffels   +3 more
doaj   +1 more source

Phylogeny based discovery of regulatory elements [PDF]

BMC Bioinformatics, 2006
Abstract Background Algorithms that locate evolutionarily conserved sequences have become powerful tools for finding functional DNA elements, including transcription factor binding sites; however, most methods do not take advantage of an explicit model for the constrained evolution of functional DNA sequences.
Gertz, Jason, Fay, Justin C, Cohen, Barak A   +2 more
openaire   +4 more sources

Remotely acting SMCHD1 gene regulatory elements: in silico prediction and identification of potential regulatory variants in patients with FSHD [PDF]

, 2015
Background: Facioscapulohumeral dystrophy (FSHD) is commonly associated with contraction of the D4Z4 macro-satellite repeat on chromosome 4q35 (FSHD1) or mutations in the SMCHD1 gene (FSHD2).
B Mifsud, C Huichalaf, C Wijmenga, E Lieberman-Aiden, I Dunham, J Dekker, J Winston, KS Pollard, L Daxinger, LM Hartweck, M Larsen, M Richards, M Upadhyaya, MP Creyghton, N Harmston, R Andersson, RE Thurman, RJ Lemmers, RJ Lemmers, RJ Lemmers, S Bhatia, S Sacconi, SE Hamby, SS Rao, TI Jones, W Zeng, Z Yao   +26 more
core   +2 more sources

Widespread Long-range Cis-Regulatory Elements in the Maize Genome

Nature Plants, 2019
Genetic mapping studies on crops suggest that agronomic traits can be controlled by gene–distal intergenic loci. Despite the biological importance and the potential agronomic utility of these loci, they remain virtually uncharacterized in all crop ...
William A Ricci, Zefu Lu, Lexiang Ji, Alexandre P. Marand, Christina L. Ethridge, N. Murphy, Jaclyn M. Noshay, M. Galli, M. K. Mejía-Guerra, M. Colomé-Tatché, F. Johannes, M. Rowley, V. Corces, J. Zhai, M. Scanlon, E. Buckler, A. Gallavotti, Nathan M. Springer, Robert J. Schmitz, Robert J. Schmitz, Xiaoyu Zhang   +20 more
semanticscholar   +1 more source

Reporter constructs with low background activity utilizing the cat gene [PDF]

, 1992
Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements ...
Andrea Schmidt, Boshart, Bruno Luckow, Buchman, Günther Schütz, Heard, Luckow, Michael Boshart, Michael Klüppel, Ruppert, Schüle, Seamon   +11 more
core   +1 more source