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Regulatory Sequences of Pear [PDF]
, 2019 Pear (Pyrus) is one of the leading and oldest cultivated fruit trees of temperate regions that is grown around the world. Compared to other Rosaceae species, pear research studies have lagged behind other members of the Rosaceae, such as strawberry, peach, and apple.
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In pursuit of design principles of regulatory sequences
Nature Reviews Genetics, 2014Instructions for when, where and to what level each gene should be expressed are encoded within regulatory sequences. The importance of motifs recognized by DNA-binding regulators has long been known, but their extensive characterization afforded by recent technologies only partly accounts for how regulatory instructions are encoded in the genome. Here,
Michal Levo, Eran Segal
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Evolution of cis-Regulatory Sequences in Drosophila
2010Cross-species comparison is an emerging paradigm for identifying cis-regulatory sequences and understanding their function and evolution. In this chapter, we review probabilistic models of evolution of transcription factor binding sites, which provide the theoretical basis for a number of new bioinformatics tools for comparative sequence analysis.
Saurabh Sinha, Xin He
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Recognition of regulatory regions in genomic sequences
Journal of Biotechnology, 1994For the functional interpretation of genomic sequences, effective algorithms have to be developed that will recognize regions of specific function and thus will suggest experiments for their verification. As a first step, relevant data have to be collected in an appropriate database from which suitable training sets can be extracted.
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Genes and regulatory sequences of bacteriophage ∅X174
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1983Fragments of the DNA of bacteriophage phi X174 were inserted in the plasmids pACYC177 and pBR322, in order to test the in vivo effects of separate phage genes and regulatory sequences. The phi X174 inserts were identified by recombination and complementation with phage mutants, followed by restriction enzyme analysis.
Peter Weisbeek +3 more
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Searching for regulatory elements in human noncoding sequences
Current Opinion in Structural Biology, 1997Important progress has been made in the past two years in the identification of Pol II promoters. For most other regulatory elements, however, current biological knowledge is still insufficient to allow the development of prediction tools. The phylogenetic-footprinting strategy, which is based on the comparative analysis of homologous sequences, is a ...
Duret, L., Bucher, P.
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Characterization of human and mouse H19 regulatory sequences
Molecular Biology Reports, 2000H19 is expressed in a large percentage of bladder tumors, but not expressed in healthy bladder tissue. The aim of this study is to define H19 optimal transcriptional regulatory sequences in tumor cells, which can potentially be used to control expression of a toxin gene in constructs to be used in bladder cancer gene therapy trials in mice and human ...
Patricia Ohana +9 more
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Motif and Regulatory Sequence Analysis
2019In this chapter, we will learn and practice (1) type and method of sequence alignment (2) sequence motif searching, sequence logo creation, and phylogenetic tree analysis (3) prediction of transcription factor and microRNA (miRNA) binding sites involved in gene regulation (4) visualization and exploration of sequence annotations using a genome browser.
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Chapter 3 Evolution of Cis‐Regulatory Sequences in Drosophila
2008Altered expression of genes during development is one mechanism that might underlie morphological diversity in animals. Comparison has shown that differences in gene expression often correlate with differences in morphology between species. However, many of these examples involve slowly evolving traits between widely diverged taxa, making investigation
Savita Ayyar, Pat Simpson
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Conservation of sequence and function in the Pax6 regulatory elements
Trends in Genetics, 2004Abstract The Pax6 transcription factor is a highly conserved regulator of eye and brain development. Its complex mode of regulation is shared between Drosophila melanogaster and the vertebrates, despite there being no significant sequence identity between their enhancer and promoter regions. In this article, a more detailed examination of the Pax6
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