Results 61 to 70 of about 1,518,123 (337)
FEBS Letters, EarlyView.In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
Nature CommunicationsRegulatory sequences encode crucial gene expression signals, yet the sequence characteristics that determine their functionality across species remain obscure.
Pengcheng Zhang +4 more
doaj +1 more source
MEIRLOP: improving score-based motif enrichment by incorporating sequence bias covariates
BMC Bioinformatics, 2020 Background Motif enrichment analysis (MEA) identifies over-represented transcription factor binding (TF) motifs in the DNA sequence of regulatory regions, enabling researchers to infer which transcription factors can regulate transcriptional response to ...
Nathaniel P. Delos Santos +2 more
doaj +1 more source
CastNet: a systems-level sequence evolution simulator
BMC Bioinformatics, 2023 Background Simulating DNA evolution has been done through coevolution-agnostic probabilistic frameworks for the past 3 decades. The most common implementation is by using the converse of the probabilistic approach used to infer phylogenies which, in the ...
Carlos J. Rivera-Rivera, Djordje Grbic
doaj +1 more source
Learning the Regulatory Code of Gene Expression
Frontiers in Molecular Biosciences, 2021 Data-driven machine learning is the method of choice for predicting molecular phenotypes from nucleotide sequence, modeling gene expression events including protein-DNA binding, chromatin states as well as mRNA and protein levels.
Jan Zrimec +6 more
doaj +1 more source
Local sequence features that influence AP-1 cis-regulatory activity [PDF]
, 2018 In the genome, most occurrences of transcription factor binding sites (TFBS) have no cis-regulatory activity, which suggests that flanking sequences contain information that distinguishes functional from nonfunctional TFBS.
Chaudhari, Hemangi G, Cohen, Barak A
core +2 more sources
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Genetics and regulatory impact of alternative polyadenylation in human B-lymphoblastoid cells.
PLoS Genetics, 2012 Gene expression varies widely between individuals of a population, and regulatory change can underlie phenotypes of evolutionary and biomedical relevance.
Oh Kyu Yoon +3 more
doaj +1 more source
DanQ: a hybrid convolutional and recurrent deep neural network for quantifying the function of DNA sequences. [PDF]
, 2016 Modeling the properties and functions of DNA sequences is an important, but challenging task in the broad field of genomics. This task is particularly difficult for non-coding DNA, the vast majority of which is still poorly understood in terms of ...
Quang, Daniel, Xie, Xiaohui
core +2 more sources
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source