Results 81 to 90 of about 76,259 (304)

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Polymerase δ replicates both strands after homologous recombination-dependent fork restart [PDF]

, 2015
To maintain genetic stability DNA must be replicated only once and replication completed even when individual replication forks are inactivated. Because fork inactivation is common, the passive convergence of an adjacent fork is insufficient to rescue ...
A Calzada, A Deem, A Errico, A Malkova, AC Bester, AM Carr, Andrea Keszthelyi, Antony M Carr, AR Clausen, AT Watson, AT Watson, B Arcangioli, C Greenman, CE Smith, CM Carvalho, D Dominguez-Sola, DE Lea, E Petermann, GR Bignell, I Iraqui, I Miyabe, Izumi Miyabe, J Bartkova, J Bartkova, JH Barlow, Johanne M Murray, K Mizuno, K Mizuno, K Schlacher, Ken'Ichi Mizuno, KJ Neelsen, L Costantino, M Kai, M Lopes, M Meselson, MA Reijns, MA Wilson, Meliti Skouteri, MO Nguyen, MR Higgs, N Saini, P Liu, R Di Micco, RA Donnianni, RE Georgescu, RM Jones, RP Anand, S Jain, S Lambert, S Lambert, S Lambert, S Lambert, S Mohebi, S Moreno, S Waga, SA Lujan, Saed Mohebi, T Eydmann, T Handa, T Kesti, TA Kunkel, Thomas A Kunkel, VG Gorgoulis, XQ Ge, Y Daigaku, Yasukazu Daigaku, Z Sun   +66 more
core   +2 more sources

RMI1 Promotes DNA Replication Fork Progression and Recovery from Replication Fork Stress [PDF]

Molecular and Cellular Biology, 2012
RMI1 is a member of an evolutionarily conserved complex composed of BLM and topoisomerase IIIα (TopoIIIα). This complex exhibits strand passage activity in vitro, which is likely important for DNA repair and DNA replication in vivo. The inactivation of RMI1 causes genome instability, including elevated levels of sister chromatid exchange and ...
Jay, Yang, Lara, O'Donnell, Daniel, Durocher, Grant W, Brown   +3 more
openaire   +2 more sources

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Plasmid DNA replication and topology as visualized by two-dimensional agarose gel electrophoresis [PDF]

, 2010
31 p.- 12 fig.During the last 20 years, two-dimensional agarose gel electrophoresis combined with other techniques such as Polymerase Chain Reaction, helicase assay and electron microscopy, helped to characterize plasmid DNA replication and topology ...
Krimer, Dora B., Martínez-Robles, María Luisa, Schvartzman, Jorge Bernardo   +2 more
core   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Reversion from basal histone H4 hypoacetylation at the replication fork increases DNA damage in FANCA deficient cells.

PLoS ONE
The FA/BRCA pathway safeguards DNA replication by repairing interstrand crosslinks (ICL) and maintaining replication fork stability. Chromatin structure, which is in part regulated by histones posttranslational modifications (PTMs), has a role in ...
Benilde García-de Teresa, Cecilia Ayala-Zambrano, Mirna González-Suárez, Bertha Molina, Leda Torres, Alfredo Rodríguez, Sara Frías   +6 more
doaj   +1 more source

Replication fork binding triggers structural changes in the PriA helicase that govern DNA replication restart in E. coli

Nature Communications, 2023
Bacterial replisomes often dissociate from replication forks before chromosomal replication is complete. To avoid the lethal consequences of such situations, bacteria have evolved replication restart pathways that reload replisomes onto prematurely ...
Alexander T. Duckworth, Peter L. Ducos, Sarah D. McMillan, Kenneth A. Satyshur, Katelien H. Blumenthal, Haley R. Deorio, Joseph A. Larson, Steven J. Sandler, Timothy Grant, James L. Keck   +9 more
doaj   +1 more source

Replication fork instability and the consequences of fork collisions from rereplication [PDF]

, 2016
Replication forks encounter obstacles that must be repaired or bypassed to complete chromosome duplication before cell division. Proteomic analysis of replication forks suggests that the checkpoint and repair machinery travels with unperturbed forks ...
Alexander, Jessica Lynne, Orr-Weaver, Terry   +1 more
core   +1 more source

Termination of Eukaryotic Replication Forks [PDF]

, 2017
Termination of DNA replication forks takes place when two replication forks coming from neighbouring origins meet each other usually in the midpoint of the replicon. At this stage, the remaining fragments of DNA have to be unwound, all remaining DNA replicated and newly synthesised strands ligated to produce continuous sister chromatids.
openaire   +2 more sources