Results 41 to 50 of about 3,588,418 (239)

The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans

Biology, 2023
Understanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward ...
Bik-Kwoon Tye, Yuanliang Zhai
doaj   +1 more source

Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation

EMBO Reports, 2017
The human RIF1 protein controls DNA replication, but the molecular mechanism is largely unknown. Here, we demonstrate that human RIF1 negatively regulates DNA replication by forming a complex with protein phosphatase 1 (PP1) that limits phosphorylation ...
S. Hiraga, T. Ly, J. Garzón, Z. Hořejší, Yoshinobu Ohkubo, Akinori Endo, C. Obuse, S. Boulton, A. Lamond, A. Donaldson   +9 more
semanticscholar   +1 more source

Mechanism of DNA Recognition at a Viral Replication Origin [PDF]

, 2006
Recognition of the DNA origin by the Epstein-Barr nuclear antigen 1 (EBNA1) protein is the primary event in latentphase genome replication of the Epstein-Barr virus, a model for replication initiation in eukaryotes.
de Prat Gay, Gonzalo, Frappier, Lori, Freire Espeleta, Eleonora, Oddo, Cristian   +3 more
core   +1 more source

Rapid DNA replication origin licensing protects stem cell pluripotency

bioRxiv, 2017
Complete and robust human genome duplication requires loading MCM helicase complexes at many DNA replication origins, an essential process termed origin licensing.
J. Matson, R. Dumitru, Phillip Coryell, Ryan M. Baxley, Weili Chen, K. Twaroski, Beau R. Webber, J. Tolar, A. Bielinsky, J. Purvis, J. Cook   +10 more
semanticscholar   +1 more source

G-quadruplexes of KSHV oriLyt play important roles in promoting lytic DNA replication

Microbiology Spectrum, 2023
Kaposi’s sarcoma-associated herpesvirus (KSHV) utilizes distinct origins of DNA replication (origin of replications) during the latent and lytic phases of its life cycle.
Prerna Dabral, Timsy Uppal, Subhash C. Verma   +2 more
doaj   +1 more source

Cryo-EM structure of a licensed DNA replication origin

Nature Communications, 2017
Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM
F. Abid Ali, M. Douglas, J. Locke, V. E. Pye, A. Nans, J. Diffley, Alessandro Costa   +6 more
semanticscholar   +1 more source

Damage-induced phosphorylation of Sld3 is important to block late origin firing. [PDF]

, 2010
Origins of replication are activated throughout the S phase of the cell cycle such that some origins fire early and others fire late to ensure that each chromosome is completely replicated in a timely fashion.
Defazio-Eli, Lisa G, Jonsson, Zophonias O, Lopez-Mosqueda, Jaime, Maas, Nancy L, Toczyski, David P, Wohlschlegel, James   +5 more
core   +2 more sources

Low Affinity DnaA-ATP Recognition Sites in E. coli oriC Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication

Frontiers in Microbiology, 2018
Although the mechanisms that precisely time initiation of chromosome replication in bacteria remain unclear, most clock models are based on accumulation of the active initiator protein, DnaA-ATP.
Prassanna Rao, Tania A. Rozgaja, Abdulaziz Alqahtani, Julia E. Grimwade, Alan C. Leonard   +4 more
doaj   +1 more source

Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication

eLife, 2020
DNA replication is needed to duplicate a cell’s genome in S phase and segregate it during cell division. Previous work in Leishmania detected DNA replication initiation at just a single region in each chromosome, an organisation predicted to be ...
Jeziel Dener Damasceno, Catarina A Marques, Dario Beraldi, Kathryn Crouch, Craig Lapsley, Ricardo Obonaga, Luiz RO Tosi, Richard McCulloch   +7 more
doaj   +1 more source

Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure. [PDF]

PLoS Genetics, 2010
Eukaryotic DNA replication origins differ both in their efficiency and in the characteristic time during S phase when they become active. The biological basis for these differences remains unknown, but they could be a consequence of chromatin structure ...
Nicolas M Berbenetz, Corey Nislow, Grant W Brown   +2 more
doaj   +1 more source