Results 31 to 40 of about 1,971,949 (299)

The Macromolecular Machines that Duplicate the Escherichia coli Chromosome as Targets for Drug Discovery

Antibiotics, 2018
DNA replication is an essential process. Although the fundamental strategies to duplicate chromosomes are similar in all free-living organisms, the enzymes of the three domains of life that perform similar functions in DNA replication differ in amino ...
Jon M. Kaguni
doaj   +1 more source

Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication

eLife, 2020
DNA replication is needed to duplicate a cell’s genome in S phase and segregate it during cell division. Previous work in Leishmania detected DNA replication initiation at just a single region in each chromosome, an organisation predicted to be ...
Jeziel Dener Damasceno, Catarina A Marques, Dario Beraldi, Kathryn Crouch, Craig Lapsley, Ricardo Obonaga, Luiz RO Tosi, Richard McCulloch   +7 more
doaj   +1 more source

Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure. [PDF]

PLoS Genetics, 2010
Eukaryotic DNA replication origins differ both in their efficiency and in the characteristic time during S phase when they become active. The biological basis for these differences remains unknown, but they could be a consequence of chromatin structure ...
Nicolas M Berbenetz, Corey Nislow, Grant W Brown   +2 more
doaj   +1 more source

Dynamic relocalization of replication origins by Fkh1 requires execution of DDK function and Cdc45 loading at origins

eLife, 2019
Chromosomal DNA elements are organized into spatial domains within the eukaryotic nucleus. Sites undergoing DNA replication, high-level transcription, and repair of double-strand breaks coalesce into foci, although the significance and mechanisms giving ...
Haiyang Zhang, Meghan V Petrie, Yiwei He, Jared M Peace, Irene E Chiolo, Oscar M Aparicio   +5 more
doaj   +1 more source

Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro [PDF]

, 2010
Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs).
Sercombe, Heather E, Ainscough, Justin F X, Coverley, Dawn, Copeland, Nikki A   +3 more
core   +4 more sources

DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts. [PDF]

, 2002
Alkylating agents, such as methyl methanesulfonate (MMS), damage DNA and activate the DNA damage checkpoint. Although many of the checkpoint proteins that transduce damage signals have been identified and characterized, the mechanism that senses the ...
Lindsay, Howard D., Stokes, Matthew P., Van Hatten, Ruth, Howard D. Lindsay (4252681), Howard D. Lindsay, W. Matthew Michael, Ruth Van Hatten (16043837), W. Matthew Michael (3211092), Ruth Van Hatten, Matthew P. Stokes, Matthew P. Stokes (9707794), Michael, W. Matthew   +11 more
core   +5 more sources

Ori-Finder 2022: A Comprehensive Web Server for Prediction and Analysis of Bacterial Replication Origins

Genomics, Proteomics & Bioinformatics, 2022
The replication of DNA is a complex biological process that is essential for life. Bacterial DNA replication is initiated at genomic loci referred to as replication origins (oriCs).
Mei-Jing Dong, Hao Luo, Feng Gao
doaj   +1 more source

Identification of 1600 replication origins in S. cerevisiae

eLife
There are approximately 500 known origins of replication in the yeast genome, and the process by which DNA replication initiates at these locations is well understood.
Eric J Foss, Carmina Lichauco, Tonibelle Gatbonton-Schwager, Sara J Gonske, Brandon Lofts, Uyen Lao, Antonio Bedalov   +6 more
doaj   +1 more source

Universal temporal profile of replication origin activation in eukaryotes. [PDF]

PLoS ONE, 2009
Although replication proteins are conserved among eukaryotes, the sequence requirements for replication initiation differ between species. In all species, however, replication origins fire asynchronously throughout S phase. The temporal program of origin
Arach Goldar, Marie-Claude Marsolier-Kergoat, Olivier Hyrien   +2 more
doaj   +1 more source

Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels [PDF]

, 2010
In the metazoan replication timing program, clusters of replication origins located in different subchromosomal domains fire at different times during S phase. We have used Xenopus laevis egg extracts to drive an accelerated replication timing program in
Blow, J. Julian; id_orcid, Thomson, Alexander M., Gillespie, Peter J., Peter J. Gillespie, J. Julian Blow, Blow, J. Julian, Alexander M. Thomson   +6 more
core   +1 more source