Results 41 to 50 of about 1,971,949 (299)

Comprehensive Analysis of Replication Origins in Saccharomyces cerevisiae Genomes

Frontiers in Microbiology, 2019
DNA replication initiates from multiple replication origins (ORIs) in eukaryotes. Discovery and characterization of replication origins are essential for a better understanding of the molecular mechanism of DNA replication. In this study, the features of
Dan Wang, Feng Gao, Feng Gao, Feng Gao
doaj   +1 more source

DNA replication licensing in somatic and germ cells [PDF]

, 2004
The DNA replication (or origin) licensing system ensures precise duplication of the genome in each cell cycle and is a powerful regulator of cell proliferation in metazoa.
Williams, C, Jung, Hyo-Il, Obermann, EC, Ellen C. Obermann, Kai Stoeber, Loddo, Marco, Kathryn Leigh Eward, Marco Loddo, Shreeram, S, Williams, GH, Obermann, Ellen C, Hyo-Il Jung, Williams, Craig, J. Julian Blow, Gareth H. Williams, Eward, Kathryn Leigh, Fanshawe, Thomas, Jung, HI, Shreeram, S., Prevost, Toby; id_orcid, Blow, J. Julian; id_orcid, A. Toby Prevost, Loddo, M, Williams, Gareth H., Thomas Fanshawe, Fanshawe, T, Obermann, Ellen C., Eward, KL, Williams, Gareth H, Jung, H-I, S. Shreeram, Blow, J Julian, Prevost, A. Toby, Stoeber, K, Stoeber, Kai, Blow, J. Julian, Prevost, AT, Blow, JJ, Craig Williams   +38 more
core   +1 more source

Molecular Characterization of the Dbf4/Drf1-Dependent Kinase (DDK) and the DNA Replication Checkpoint Mediator Claspin in Xenopus Egg Extracts [PDF]

, 2010
The integrity of DNA replication control and checkpoint mechanisms is essential for preventing tumorigenesis. DNA replication is initiated by the S-CDK and DDK kinases which mediate the unwinding of the replication fork.
Gold, Daniel A.
core   +1 more source

Primosomal protein PriC rescues replication initiation stress by bypassing the DnaA-DnaB interaction step for DnaB helicase loading at oriC

eLife
In Escherichia coli, replisome and replication fork assembly is initiated by DnaB helicase loading at the chromosomal origin oriC via its interactions with the DnaA initiator and the DnaC helicase loader.
Ryusei Yoshida, Kazuma Korogi, Qinfei Wu, Shogo Ozaki, Tsutomu Katayama   +4 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Molecular Architecture of the DNA Replication Origin Activation Checkpoint [PDF]

, 2010
Perturbation of DNA replication initiation arrests human cells in G1, pointing towards an origin activation checkpoint. We used RNAi against Cdc7 kinase to inhibit replication initiation and dissect this checkpoint in fibroblasts.
Claire Mulvey, Slavica Tudzarova, Kai Stoeber, Tudzarova, S, Mulvey, C, Godovac-Zimmermann, J, Wollenschlager, A, Jasminka Godovac-Zimmermann, Matthew W B Trotter, Alex Wollenschlaeger, Stoeber, K, Gareth H Williams, Trotter, MWB, Williams, G   +13 more
core   +1 more source

A back-up mechanism for replication

eLife
A protein called PriC allows DNA replication to proceed in Escherichia coli when the complex that usually initiates this process is compromised.
Godefroid Charbon, Anders Løbner-Olesen
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Dynamic distribution of SeqA protein across the chromosome of Escherichia coli K-12 [PDF]

, 2010
The bacterial SeqA protein binds to hemi-methylated GATC sequences that arise in newly synthesized DNA upon passage of the replication machinery. In Escherichia coli K-12, the single replication origin oriC is a well-characterized target for SeqA, which ...
Stephen J. W. Busby, Nigel P. Dyer, Sascha Ott, Sánchez-Romero, María Antonia, Dyer, NP, Millard, AD, Sanchez-Romero, MA, María Antonia Sánchez-Romero, Busby, Stephen; id_orcid, Ott, S, Grainger, David, Dyer, Nigel, Millard, Andrew D., David C. Grainger, Ott, Sascha, Andrew D. Millard, Busby, S. (Stephen), Grainger, David C.   +17 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source