Results 21 to 30 of about 247,162 (311)

The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]

, 2012
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Blow, J. Julian; id_orcid, Remi Sonneville, Matthieu Querenet, Sonneville, Remi, J. Julian Blow, Anton Gartner, Querenet, Matthieu, Blow, J. Julian, Gartner, Anton, Craig, Ashley, Ashley Craig   +10 more
core   +1 more source

The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans

Biology, 2023
Understanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward ...
Bik-Kwoon Tye, Yuanliang Zhai
doaj   +1 more source

Concurrent Replication and Methylation at Mammalian Origins of Replication [PDF]

Molecular and Cellular Biology, 1998
Observations made with Escherichia coli have suggested that a lag between replication and methylation regulates initiation of replication. To address the question of whether a similar mechanism operates in mammalian cells, we have determined the temporal relationship between initiation of replication and methylation in mammalian cells both at a ...
F D, Araujo, J D, Knox, M, Szyf, G B, Price, M, Zannis-Hadjopoulos   +4 more
openaire   +2 more sources

Nucleosomes influence multiple steps during replication initiation

eLife, 2017
Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA replication assays, we assessed the impact of nucleosomes on replication initiation.
Ishara F Azmi, Shinya Watanabe, Michael F Maloney, Sukhyun Kang, Jason A Belsky, David M MacAlpine, Craig L Peterson, Stephen P Bell   +7 more
doaj   +1 more source

Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses [PDF]

, 2002
The generation of deletion mutants, including defective interfering viruses, upon serial passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in insect cell culture has been studied. Sequences containing the non-homologous region origin
Pijlman, G.P., Yang, K., Martens, D.E., Goldbach, R.W., Vlak, J.M., Dortmans, Jos C. F. M., Vermeesch, Angela M. G., Goldbach, Rob W., Martens, Dirk E., Pijlman, Gorben P., Yang, Kai, Vlak, Just M., Dortmans, J.C.F.M., Vermeesch, A.M.G.   +13 more
core   +1 more source

Replication control: Choreographing replication origins [PDF]

Current Biology, 1998
Budding yeast replication origins are activated during S phase according to a predetermined temporal programme. Two recent studies indicate that this programme is executed, at least in part, by the S-phase-promoting cyclins that act to assemble a pre-initiation complex which includes the Cdc45 protein.
openaire   +2 more sources

Low Affinity DnaA-ATP Recognition Sites in E. coli oriC Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication

Frontiers in Microbiology, 2018
Although the mechanisms that precisely time initiation of chromosome replication in bacteria remain unclear, most clock models are based on accumulation of the active initiator protein, DnaA-ATP.
Prassanna Rao, Tania A. Rozgaja, Abdulaziz Alqahtani, Julia E. Grimwade, Alan C. Leonard   +4 more
doaj   +1 more source

G-quadruplexes of KSHV oriLyt play important roles in promoting lytic DNA replication

Microbiology Spectrum, 2023
Kaposi’s sarcoma-associated herpesvirus (KSHV) utilizes distinct origins of DNA replication (origin of replications) during the latent and lytic phases of its life cycle.
Prerna Dabral, Timsy Uppal, Subhash C. Verma   +2 more
doaj   +1 more source

Genome organization of DNA replication timing and its link to chromatin and transcription [PDF]

, 2008
The replication of the genome is a highly organized process. Not every sequence replicates at the same time, instead some genes replicate early, while others replicate later during S phase.
Schwaiger, Michaela
core   +1 more source

The Macromolecular Machines that Duplicate the Escherichia coli Chromosome as Targets for Drug Discovery

Antibiotics, 2018
DNA replication is an essential process. Although the fundamental strategies to duplicate chromosomes are similar in all free-living organisms, the enzymes of the three domains of life that perform similar functions in DNA replication differ in amino ...
Jon M. Kaguni
doaj   +1 more source