Results 91 to 100 of about 34,336 (231)
License to Replicate: Mechanisms of Licensing Eukaryotic Origins for DNA Replication
BioEssays, Volume 48, Issue 1, January 2026.Origin licensing constitutes the first step in initiating eukaryotic genome replication. Licensed origins are marked by an MCM double hexamer complex. This article reviews the molecular mechanisms of MCM double hexamer loading in yeast and higher eukaryotes, based on insights gained from biochemical reconstitution and structural analyses.
Victoria Frisbie, Franziska Bleichert
wiley +1 more source
Cell Reports, 2017 Disassembly of the Cdc45-MCM-GINS (CMG) DNA helicase is the key regulated step during DNA replication termination in eukaryotes, involving ubiquitylation of the Mcm7 helicase subunit, leading to a disassembly process that requires the Cdc48 “segregase ...
Marija Maric +4 more
doaj +1 more source
Template-directed biopolymerization: tape-copying Turing machines
, 2012 DNA, RNA and proteins are among the most important macromolecules in a living cell. These molecules are polymerized by molecular machines. These natural nano-machines polymerize such macromolecules, adding one monomer at a time, using another linear ...
Chowdhury, Debashish, Sharma, Ajeet K.
core +1 more source
A requirement for STAG2 in replication fork progression creates a targetable synthetic lethality in cohesin-mutant cancers. [PDF]
, 2019 Cohesin is a multiprotein ring that is responsible for cohesion of sister chromatids and formation of DNA loops to regulate gene expression. Genomic analyses have identified that the cohesin subunit STAG2 is frequently inactivated by mutations in cancer.
Ashworth, Alan +4 more
core +2 more sources
CRISPR‐DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution
Advanced Science, Volume 12, Issue 45, December 4, 2025.This study develops an in vivo targeted mutagenesis system based on CRISPR‐guided bacteriophage T5 or T7 DNA polymerases. This system can enable all possible types of substitution mutations within an expanded mutagenesis window in Escherichia coli and exhibit application potential in different microorganisms, thus accelerating the evolution of ...
Shuaili Chen +8 more
wiley +1 more source
A Decade of Discovery—Eukaryotic Replisome Disassembly at Replication Termination
BiologySimple Summary During cell division, DNA is duplicated through a process called DNA replication, so that each new cell inherits a copy of its own. A high level of accuracy is essential in this for the maintenance of genome stability and the prevention of
Rebecca M Jones +2 more
semanticscholar +1 more source
The Eukaryotic Replisome Goes Under the Microscope [PDF]
Current Biology, 2016 The machinery at the eukaryotic replication fork has seen many new structural advances using electron microscopy and crystallography. Recent structures of eukaryotic replisome components include the Mcm2-7 complex, the CMG helicase, DNA polymerases, a Ctf4 trimer hub and the first look at a core replisome of 20 different proteins containing the ...
O’Donnell, Mike, Li, Huilin
openaire +2 more sources
From Fluctuations to Function: Unveiling the Role of Stochasticity in Life‐Like Systems
ChemSystemsChem, Volume 7, Issue 6, November 2025.In this short review, we put forward the idea that randomly fluctuating, or stochastic, molecular events can and do play a role in the emergence of complex biological phenomena at longer length scales. There is a growing body of evidence of this occurring in natural systems, and we survey the computational and experimental attempts of researchers to ...
Aleksa Lakic, Alexander F. Mason
wiley +1 more source
Approaching Protein Barriers: Emerging Mechanisms of Replication Pausing in Eukaryotes
Frontiers in Cell and Developmental Biology, 2021 During nuclear DNA replication multiprotein replisome machines have to jointly traverse and duplicate the total length of each chromosome during each cell cycle. At certain genomic locations replisomes encounter tight DNA-protein complexes and slow down.
Maksym Shyian, David Shore
doaj +1 more source
The causes and consequences of topological stress during DNA replication [PDF]
, 2016 The faithful replication of sister chromatids is essential for genomic integrity in every cell division. The replication machinery must overcome numerous difficulties in every round of replication, including DNA topological stress.
Andrea Keszthelyi +7 more
core +2 more sources