Results 161 to 170 of about 7,873,006 (309)

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Development of a Novel Human Hepatoma Cell Line Supporting the Replication of a Recombinant HBV Genome with a Reporter Gene. [PDF]

Viruses
Kawase S, Shimakami T, Kuroki K, Murai K, Funaki M, Yoshita M, Kakuya M, Suzuki R, Li YY, Gantumur D, Kawane T, Matsumori K, Nio K, Kawaguchi K, Takatori H, Honda M, Yamashita T.   +16 more
europepmc   +1 more source

Reporter gene assays and chromatin-level assays define substantially non-overlapping sets of enhancer sequences. [PDF]

BMC Genomics, 2023
Lindhorst D, Halfon MS.
europepmc   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Advancements in the application of reporter gene cell lines in bioactivity evaluation of biological products. [PDF]

Bioresour Bioprocess
Yi K, Wang C, Lu H.
europepmc   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette in <i>Sulfolobus acidocaldarius</i>. [PDF]

Front Microbiol
Xu Y, Peeters AI, Bervoets I, De Mey M, Baes R, Peeters E.   +5 more
europepmc   +1 more source

Identifying CAR Modulators Utilizing a Reporter Gene Assay. [PDF]

Methods Mol Biol, 2022
Lynch C, Zhao J, Wang H, Xia M.
europepmc   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Three isoforms of <i>Brugia malayi</i> <i>daf-16</i> activate a reporter gene in cultured HepG2 cells. [PDF]

MicroPubl Biol
Zehr CE, Folk A, Stanford K, Maiorelle J, Crossgrove K.   +4 more
europepmc   +1 more source