Results 201 to 210 of about 357,104 (247)

Reporter Gene Imaging

American Journal of Roentgenology, 2013
The purposes of this article are to summarize the basic concept and the strategies of reporter imaging; introduce reporter genes frequently used in optical imaging, nuclear medicine, and MRI for in vivo application; and show typical examples of reporter gene imaging.In molecular biology, many reporter genes have been developed for monitoring cellular ...
Hyewon, Youn, June-Key, Chung
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Luciferases as Reporter Genes

2006
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern, Paul E, Brown, Anthony, Hall   +2 more
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Reporter gene expression for monitoring gene transfer

Current Opinion in Biotechnology, 1997
The use of reporters such as green fluorescent protein (GFP) and firefly luciferase permit highly sensitive and nondestructive monitoring of gene transfer and expression. Modifications in GFP which increase intensity and thermostability, as well as alter its spectral qualities, have facilitated the use of GFP in a variety of gene transfer methods ...
S, Welsh, S A, Kay
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Monitoring gene therapy with reporter gene imaging

Seminars in Nuclear Medicine, 2001
Rapid advances in imaging technologies and gene transfer strategies offer a great opportunity to optimize clinical trials of human gene therapy. Reporter genes are emerging as very powerful tools to monitor the delivery, magnitude, and time variation of therapeutic gene transfer in vivo.
P, Ray, E, Bauer, M, Iyer, J R, Barrio, N, Satyamurthy, M E, Phelps, H R, Herschman, S S, Gambhir   +7 more
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A Reporter Gene to Analyse the Hypermutation of Immunoglobulin Genes

Journal of Molecular Biology, 1995
The affinity maturation of antibodies is driven by somatic hypermutation which is localized to specific segments of the coding genes. The information available on this process derives from studies in vivo. With the intention of developing new approaches, we have constructed a fusion gene between a kappa chain and a selectable neomycin resistance gene ...
Y L, Chui, F, Lozano, J M, Jarvis, R, Pannell, C, Milstein   +4 more
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Aequorin as a Reporter Gene

2006
Reporter proteins allow one to monitor cellular parameters that are involved in signal transduction, development, metabolic processes, and transport. There are targeting strategies available to direct the indicator protein exactly to the locale inside the organism from which information is desired.
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Reporter Gene Vectors and Assays

Molecular Biotechnology, 1999
Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems.
E, Schenborn, D, Groskreutz
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Genes and Development—A Workshop Report

Placenta, 2004
The ‘Genes and Development’ workshop focused on recentadvances in our understanding of the molecular control ofplacental development, focusing on the mouse model. JayCross gave an introduction to mouse placental developmentthat outlined the major structures and cell types. He alsoreviewed the considerable progress that has been made indefining molecular
J C, Cross, P M, Coan, R, Fundele, M, Hemberger, M, Kibschull, A, Ferguson-Smith   +5 more
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Reporter genes in transgenic mice

Transgenic Research, 1994
Although in vivo models utilizing endogenous reporter genes have been exploited for many years, the use of reporter transgenes to dissect biological issues in transgenic animals has been a relatively recent development. These transgenes are often, but not always, of prokaryotic origin and encode products not normally associated with eukaryotic cells ...
C, Cui, M A, Wani, D, Wight, J, Kopchick, P J, Stambrook   +4 more
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Tetracysteine as a Reporter for Gene Therapy

Biomedical and Environmental Sciences, 2009
To study the feasibility of using tetracysteine (TC) reporter in gene therapy.Effects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot.
Xu, Chen-Yu, Gu, Ying, Hou, Wang-Heng, Que, Yu-Qiong, Gao, Shuang-Guan, Cheng, Tong, Xia, Ning-Shao   +6 more
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