Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
Microbiology Spectrum, 2021 Recombinant viruses expressing reporter genes allow visualization and quantification of viral infections and can be used as valid surrogates to identify the presence of the virus in infected cells and animal models.
Kevin Chiem +7 more
doaj +1 more source
, 2009 Reporter gene assays are versatile and sensitive methods of assaying numerous targets in high-throughput drug-screening programs. A variety of reporter genes allow users a choice of signal that can be tailored to the required sensitivity, the available detection apparatus, the cellular system employed, and the required compatibility with multiplexed ...
Liu, A.M.F. +3 more
openaire +3 more sources
Molecular Therapy: Nucleic Acids, 2013 Despite the development of potent siRNAs that effectively target genes responsible for skin disorders, translation to the clinic has been hampered by inefficient delivery through the stratum corneum barrier and into the live cells of the epidermis ...
Robyn P Hickerson +9 more
doaj +1 more source
Adenovirus-mediated gene transfer in primary murine adipocytes
Journal of Lipid Research, 2000 The transfer of genes into primary murine adipocytes using an adenovirus system has been developed. A recombinant adenovirus was constructed (expressing green fluorescent protein [GFP] under the control of the strong cytomegalovirus [CMV] promoter and a ...
Ann Vogel Hertzel +2 more
doaj +1 more source
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
Environmental Application of Reporter-Genes Based Biosensors for Chemical Contamination Screening
Archives of Environmental Protection, 2014 The paper presents results of research concerning possibilities of applications of reporter-genes based microorganisms, including the selective presentation of defects and advantages of different new scientific achievements of methodical solutions in ...
Matejczyk Marzena +1 more
doaj +1 more source
Development Of An Engineered Bioluminescent Reporter Phage For Detection Of Bacterial Blight Of Crucifers [PDF]
, 2012 Bacterial blight, caused by the phytopathogen Pseudomonas cannabina pv. alisalensis, is an emerging disease afflicting important members of the Brassicaceae family.
Bull, Carolee T. +5 more
core +1 more source
Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo [PDF]
, 2008 We dissect the transcriptional regulatory relationships coordinating the dynamic expression patterns of two signaling genes, wnt8 and delta, which are central to specification of the sea urchin embryo endomesoderm.
Alifragis +34 more
core +3 more sources
Identification and characterization of novel factors that act in the nonsense-mediated mRNA decay pathway in nematodes, flies and mammals [PDF]
, 2014 Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome-wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD ...
Ailion M +8 more
core +2 more sources
Gcn4p and novel upstream activating sequences regulate targets of the unfolded protein response. [PDF]
, 2004 Eukaryotic cells respond to accumulation of unfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR), a signal transduction pathway that communicates between the ER and the nucleus.
Li, Hao +2 more
core +4 more sources