Results 11 to 20 of about 542,183 (294)
Non-invasive imaging using reporter genes altering cellular water permeability
Nature Communications, 2016 Magnetic resonance imaging combined with molecular reporters can visualise cellular functions in intact organisms. Here Mukherjeeet al. present a cellular imaging approach based on intracellular changes in water diffusion using human aquaporin 1 gene as ...
Arnab Mukherjee +3 more
doaj +2 more sources
Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection. [PDF]
PLoS ONE, 2018 Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and for isolation of specific cell populations for study.
Valentin M Sluch +5 more
doaj +2 more sources
The ChIP-seq-defined networks of Bcl-3 gene binding support its required role in skeletal muscle atrophy [PDF]
, 2012 NF-kappaB transcriptional activation is required for skeletal muscle disuse atrophy. We are continuing to study how the activation of NF-kB regulates the genes that encode the protein products that cause atrophy. Using ChIP-sequencing we found that Bcl-3,
Jackman, Robert W. +2 more
core +16 more sources
Frontiers in Cellular and Infection Microbiology, 2022 CRISPR editing has enabled the rapid creation of fluorescent Plasmodium transgenic lines, facilitating a deeper understanding of parasite biology. The impact of genetic perturbations such as gene disruption or the introduction of drug resistance alleles ...
Johanna Hoshizaki +2 more
doaj +1 more source
StORF-Reporter: finding genes between genes
Nucleic Acids Research, 2022 Abstract Large regions of prokaryotic genomes are currently without any annotation, in part due to well-established limitations of annotation tools. For example, it is routine for genes using alternative start codons to be misreported or completely omitted.
Nicholas J Dimonaco +4 more
openaire +4 more sources
Photoacoustic reporter genes for noninvasive molecular imaging and theranostics [PDF]
Journal of Innovative Optical Health Sciences, 2020 Noninvasive molecular imaging makes the observation and comprehensive understanding of complex biological processes possible. Photoacoustic imaging (PAI) is a fast evolving hybrid imaging technology enabling in vivo imaging with high sensitivity and ...
Zhao Lei +4 more
doaj +1 more source
Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
Microbiology Spectrum, 2021 Recombinant viruses expressing reporter genes allow visualization and quantification of viral infections and can be used as valid surrogates to identify the presence of the virus in infected cells and animal models.
Kevin Chiem +7 more
doaj +1 more source
Journal of Nuclear Medicine, 2008 Noninvasive molecular imaging of dynamic processes has benefited tremendously from the use of reporter genes. These genes encode for proteins that emit light, bind radiolabeled probes, or, as covered in this review, modulate MRI contrast. Reporter genes play a pivotal role in monitoring cell trafficking, gene replacement therapy, protein-protein ...
Assaf A, Gilad +5 more
openaire +2 more sources
Genetic Manipulation of Non-Falciparum Human Malaria Parasites
Frontiers in Cellular and Infection Microbiology, 2021 The development of genetic manipulation of Plasmodium falciparum in the 1980s was key to study malaria biology. Genetically modified parasites have been used to study several aspects of the disease, such as red blood cell invasion, drug resistance ...
Taís Baruel Vieira +2 more
doaj +1 more source
Covalent 18F-Radiotracers for SNAPTag: A New Toolbox for Reporter Gene Imaging
Pharmaceuticals, 2021 There is a need for versatile in vivo nuclear imaging reporter systems to foster preclinical and clinical research. We explore the applicability of the SNAPTag and novel radiolabeled small-molecule ligands as a versatile reporter gene system for in vivo ...
Sophie Stotz +4 more
doaj +1 more source