Results 71 to 80 of about 249,173 (312)

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

User-oriented requirements engineering [PDF]

2014 IEEE 2nd International Workshop on Usability and Accessibility Focused Requirements Engineering (UsARE), 2014
A method for analysing emotion and motivation in requirements engineering (RE) is described. The method extends personal RE where requirements are for individual users and their needs. Theories from the psychology of emotion and motivation are introduced and applied in a top-down pathway motivated by system goals to influence users, and a bottom-up ...
openaire   +2 more sources

Successful Requirement Elicitation by Combining Requirement Engineering Techniques

, 2008
Mishra, Alok/0000-0003-1275-2050; Mishra, Deepti/0000-0001-5144-3811The role of customers and other stakeholders is becoming increasingly significant during requirement engineering activities.
Mıshra, Deepti, Yazici, Ali, Mishra, Deepti, Mishra, Alok, Yazıcı, Ali, Mıshra, Alok   +5 more
core  

Sustainability Education in Software Engineering: Experiences from Requirement Engineering Curriculum

The current software engineering workforce lacks an understanding of ways to tackle the different sustainability impacts of software solutions on users, society, and the environment.
Adisa, Mikhail Ola, Oyedeji, Shola, Porras, Jari   +2 more
core   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Data management in balance – a decade of balancing pragmatism, sustainability and innovation at plant research center IPK Gatersleben

Journal of Integrative Bioinformatics
The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben is a leading international plant science institute specializing in biodiversity and crop plant performance research.
Schüler Danuta, Lange Matthias, Altmann Thomas, Cuacos Maria, Arend Daniel, D’Auria John Charles, Fiebig Anne, Kumlehn Jochen, Neumann Kerstin, Melzer Michael, Rey-Mazón Elena, Rolletschek Hardy, Scholz Uwe, Willner Evelin, Reif Jochen C.   +14 more
doaj   +1 more source

A Study of Emotions in Requirements Engineering [PDF]

, 2010
Proceedings of: 3rd World Summit on the Knowledge Society (WKKS 2010), September 22-24, 2010, Corfu (Greece) Requirements engineering (RE) is a crucial activity in software development projects. This phase in the software development cycle is knowledge intensive, and thus, human capital intensive.
Ricardo Colomo-Palacios, Adrián Hernández-López, Ángel García-Crespo, Pedro Soto-Acosta   +3 more
openaire   +2 more sources

Using software specification methods for measurement instruments: Part I - structured methods [PDF]

, 1992
Investigation has been undertaken into the use of software specification methods for instrument systems specification. In the first part of this paper, some of the well-known structured software specification methods are briefly evaluated against a set ...
B. Nuseibeh, L. Finkelstein, Finkelstein, A., A. C. W. Finkelstein, Finkelstein, L., Nuseibeh, B., J. Huang, Part Structured, Huang, J.   +8 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source