Results 61 to 70 of about 131,206,127 (300)

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Mapping the Spatiotemporal Urban Footprint of Residents and Tourists: A Data-Driven Approach Based on User-Generated Reviews

ISPRS International Journal of Geo-Information
Understanding how different population groups interact with urban environments is essential for analyzing spatial dynamics and informing urban planning, especially in cities experiencing high visitor pressure.
Mikel Barrena-Herrán, Itziar Modrego-Monforte, Olatz Grijalba   +2 more
doaj   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Dominion : an architecture-driven approach to generating efficient constraint solvers [PDF]

, 2011
This work is supported by the EPSRC grant “A Constraint Solver Synthesiser” (EP/H004092/1) and SICSA studentships.Constraints are used to solve combinatorial problems in a variety of industrial and academic disciplines.
Balasubramaniam, Dharini, Chris Jefferson, De Silva, Lakshitha Ramesh, Peter Nightingale, Dharini Balasubramaniam, Miguel, Ian James, Jefferson, Christopher Anthony, Lakshitha de Silva, Kotthoff, Lars, Nightingale, Peter, Ian Miguel, Lars Kotthoff   +11 more
core   +1 more source

Revealing Spatiotemporal Urban Activity Patterns: A Machine Learning Study Using Google Popular Times

ISPRS International Journal of Geo-Information
Extensive scientific evidence underscores the importance of identifying spatiotemporal patterns for investigating urban dynamics. The recent proliferation of location-based social networks (LBSNs) facilitates the measurement of urban rhythms through ...
Mikel Barrena-Herrán, Itziar Modrego-Monforte, Olatz Grijalba   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

From Internet Architecture Research to Standards [PDF]

, 2012
Many Internet architectural research initiatives have been undertaken over last twenty years. None of them actually reached their intended goal: the evolution of the Internet architecture is still driven by its protocols not by genuine architectural evolutions.
Dimitri Papadimitriou, Bernard Sales, Piet Demeester, Theodore B. Zahariadis   +3 more
openaire   +3 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source