Results 131 to 140 of about 12,197,949 (364)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Cross-Cultural Research Methods: Review and Prospect [PDF]

, 2010
Kwok Leung, Fan Zhou
openalex   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Modeling Method in the Scientific Research [PDF]

, 2015
Jelenka Savković‐Stevanović
openalex   +1 more source

Protocol for an economic evaluation alongside the Re-Evaluating the Inhibition of Stress Erosions (E-REVISE) trial

BMJ Open
Introduction Economic evaluations in healthcare can guide practice and inform policy. The objective of this paper is to present the protocol for a health economic evaluation comparing the cost-effectiveness of prophylactic treatment using pantoprazole 40 
Bram Rochwerg, Diane Heels-Ansdell, Deborah J Cook, Robert Fowler, Brittany Humphries, Feng Xie, Nicole Zytaruk, Vincent Lau, Yifan Yao   +8 more
doaj   +1 more source

Research methods: Module mapping

, 2010
This file is part of "Research methods" deposit. It needs to be used in conjunction with the Mapping Tool, developed as part of the social sciences subject strand OER project (see http://www.c-sap.bham.ac.uk/subject_areas/elearning/oer/default.htm).
Dr Dave Harris, MARJON
core  

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Developing methods to manage conflicts between humans and birds -- three decades of change at the USDA National Wildlife Research Center [PDF]

, 2002
E. Tobin Mark
openalex   +1 more source

Research Methods - the basics

, 2021
Research Methods: The Basics is an accessible, user-friendly introduction to the different aspects of research theory, methods and practice. This third edition provides an expanded and fully updated resource suitable for students and practitioners in a ...
Walliman, Nicholas
core