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IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS [PDF]
Journal of Cell Biology, 1961 Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenient embedding methods for electron microscopy. The sections are robust and tissue damage is less than with methacrylate embedding.
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An Epoxy Resin Embedding Technique for Large Objects
Stain Technology, 1974Undecalcified heads in sizes up to that of a mallard are embedded, sectioned (10μ), and stained according to a modification of Luft's procedure (1961). Polymerization time is prolonged by keeping the resin mixture at 4 C or by omission of the accelerator. Under these conditions embedding takes about a month.
G C, Anker +2 more
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Proceedings, annual meeting, Electron Microscopy Society of America, 1968
Since the introduction of epoxy resins as embedding material for electron microscopy, the list of new formulations and variations of widely accepted mixtures has grown rapidly. Described here is a resin system utilizing Maraglas 655, Dow D.E.R. 732, DDSA, and BDMA, which is a variation of the mixtures of Lockwood and Erlandson.
J. G. Adams +3 more
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Since the introduction of epoxy resins as embedding material for electron microscopy, the list of new formulations and variations of widely accepted mixtures has grown rapidly. Described here is a resin system utilizing Maraglas 655, Dow D.E.R. 732, DDSA, and BDMA, which is a variation of the mixtures of Lockwood and Erlandson.
J. G. Adams +3 more
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Backscattered electron imaging of resin-embedded sections
Microscopy, 2018Scanning electron microscopes have longer focal depths than transmission electron microscopes and enable visualization of the three-dimensional (3D) surface structures of specimens. While scanning electron microscopy (SEM) in biological research was generally used for the analysis of bulk specimens until around the year 2000, more recent instrumental ...
Daisuke, Koga +2 more
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Polar resin embedding for Macrotrachela quadricornifera
Hydrobiologia, 1993A new glycol methacrylate (GMA) polar resin technique was used for light microscopy and histochemistry of Macrotrachela quadricornifera. Animals were treated with cold aqueous solution of ethylene glycol (EG), then embedded in the cold. Animals required no conventional chemical fixation as EG stabilizes, dehydrates and cryopreserves their structure. In
Bruno Dore, Fausta Carnemolla
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Ultrastructural localization of a lysosomal enzyme in resin-embedded lymphocytes
Histochemistry, 1988The intracellular distribution of lysosomal enzymes in lymphocytes has previously been only poorly defined, mainly by cytochemical procedures of low resolution. In the present study we have used a post-embedding immunogold technique to identify the precise ultrastructural localization of a lysosomal enzyme, beta-glucuronidase, in activated lymphocytes ...
G, Bou-Gharios +3 more
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Epoxy Resin Embedding in Contrast Radioautography of Bones and Teeth
Stain Technology, 1960Contrast, or low-resolution, radioautographs of teeth and large specimens of bone are made from plane, polished surfaces of such material embedded in plastic. The specimen is cut with a motor-driven circular saw, fixed, dehydrated, and defatted with anhydrous acetone and ether. It is then infiltrated with a mixture of epoxy resin and reactive hardener.
W P, NORRIS, P E, JENKINS
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Microwave Procedures for Electron Microscopy and Resin-Embedded Sections
Micron, 1998Microwaves now have well-established applications in routine light microscopy. They are employed in tissue fixation and to accelerate a wide spectrum of staining procedures. Besides producing superior preservation of cellular antigens through microwave fixation, this form of irradiation has been employed for antigen retrieval, a procedure that has been
A S, Leong, R T, Sormunen
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Immunofluorescence on resin‐embedded material
Histopathology, 1977Some antigenic determinants can be preserved in tissues after plastic embedding. In the present study liver tissue was fixed with glutaraldehyde or paraformaldehyde, dehydrated with ethanol and toluene and embedded in araldite which was then polymerized at 37°C. Immunofluorescence was performed on semi‐thin sections etched with hydrogen peroxide.
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