Results 211 to 220 of about 29,160 (257)
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Resin Embedding and Immunolabelling
1993The major practical variables involved in resin embedding and immunolabelling have been dealt with in the preceding chapters, but in the discussions of these, other, more theoretical parameters were touched upon. A deeper understanding of such considerations may be important for “fine tuning” the sensitivity that can be obtained when labelling resin ...
Geoffrey R. Newman, Jan A. Hobot
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‘Routine’ stains on osmicated resin embedded hepatic tissue
Pathology, 1982The recently described modification of the osmium tetroxide method for demonstrating lipid can be used on liver biopsies taken for diagnostic purposes. An unstained section is used for the quantitation of the lipid and sequential sections can be stained by all the 'routine' liver stains.
P, Hall, R D, Smith, B M, Gormley
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Investigative Radiology, 1986
A method for simultaneous acquisition of information on cytochemical and microangiographic characteristics of tissue after perfusion with contrast medium was developed. The technique is based on the use of hydrosoluble resin as embedding material and is illustrated by the demonstration of gamma-glutamyltranspeptidase activity and of the ...
Van Dale, Patricia +3 more
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A method for simultaneous acquisition of information on cytochemical and microangiographic characteristics of tissue after perfusion with contrast medium was developed. The technique is based on the use of hydrosoluble resin as embedding material and is illustrated by the demonstration of gamma-glutamyltranspeptidase activity and of the ...
Van Dale, Patricia +3 more
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Inexpensive holder for hand‐trimming resin‐embedded tissues
Microscopy Research and Technique, 2002AbstractA simple and inexpensive mount for securely holding resin‐embedded tissues for trimming prior to sectioning for light or electron microscopy is described. The unit is composed of a base plate from which a 12.7‐mm drill chuck protrudes. A tissue block is clamped into a microtome chuck and that is mounted in the drill chuck. A standard dissecting
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Immunogold Labeling of Resin-Embedded Electron Microscopical Sections
2013Knowledge about the spatio-temporal distribution patterns of proteins and other molecules of the cell is essential for understanding their function. A widely used technique is immunolabeling which uses specific antibodies to reveal the distribution of molecular components at various structural levels.
Ilse, Foissner, Margit, Hoeftberger
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Resin embedment of organs and postembedment localization of antigens by immunoperoxidase methods
Histochemistry, 1981Various procedures for nonpolar and polar resin embedment were applied to mouse and rat livers for the study of postembedment immunolocalization of alpha 1-fetoprotein, albumin and the microsomal enzyme epoxide hydrolase. Fixations with formaldehyde and with formaldehyde-glutaraldehyde mixtures were used for tissue stabilization.
W D, Kuhlmann, R, Krischan
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Demonstration of lipids in plastic resin‐embedded sections of skin material
Journal of Microscopy, 2009SummaryTwo different fluorescence stains, green: 5‐hexadecanoylaminofluorescein, and red: BODIPY® 665/676 [(E,E)‐3, 5‐bis‐(4‐phenyl‐1,3‐butadienyl)‐4,4‐difluoro‐4‐bora‐3a, 4a‐diaza‐s‐indacene, produced good results regarding the demonstration of glycolipids, free fatty acids and triglycerides in mammalian skin material that had been embedded in a water
W, Meyer +4 more
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Polyester Resin Embedding for Sectioning of Hard and Soft Tissues
Stain Technology, 1960Soft and calcareous tissues embedded in polyester resin may be cut on a sledge microtome to produce thin sections of 3-4 β thickness.
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Improving FIB-SEM Reconstructions By Using Epoxy Resin Embedding
ECS Meeting Abstracts, 2017Focus ion beam-scanning electron microscopy (FIB-SEM) is a viable technique to visualize and reconstruct the morphology of polymer electrolyte membrane fuel cell (PEMFC) porous media, such as catalyst layers (CLs) (1,2) and micro-porous layers (MPLs) (3,4), with a high resolution of few nanometers. FIB-SEM provides morphological information about the
Mayank Sabharwal +4 more
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Improvements in Histological Techniques for Epoxy-Resin Embedded Bone Specimens
Stain Technology, 1981A procedure is presented in which some of the processing difficulties with fixation, embedding and cutting whole mouse bones and large bone pieces from other species are considered. The bone specimens are fixed in acetone or by a Karnovsky-formol-saline process which preserves intact endosteal surface-to-cortex layers.
R H, Watts, D, Green, G R, Howells
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