Results 81 to 90 of about 1,694 (248)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Use of Chitosan and Chitosan–Magnetite Spheres for Arsenic Groundwater Removal: Factorial Designs as Tools to Optimize the Efficiency of Removal

Inorganics
The lack of access to drinking water is a problem affecting many regions worldwide. In Santa Fe, Argentina, the population uses groundwater as a source of drinking water.
Mayra Hernández Trespalacios, María Florencia Mangiameli, Lina Gribaudo, María Inés Frascaroli, Juan Carlos González   +4 more
doaj   +1 more source

Predicting the Onset of Cavitation in Automotive Torque Converters—Part II: A Generalized Model

International Journal of Rotating Machinery, 2008
The objective of this investigation was to develop a dimensionless model for predicting the onset of cavitation in torque converters applicable to general converter designs.
D. L. Robinette, J. M. Schweitzer, D. G. Maddock, C. L. Anderson, J. R. Blough, M. A. Johnson   +5 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Evaluating the prediction power and accuracy of two smart response surface experimental designs after revisiting repaglinide floating tablets

Future Journal of Pharmaceutical Sciences
Background There is a soar in the figure of companies aiming to achieve efficiency in undergoing experimental processes. Therefore, instead of deploying one-factor-at-a-time, design of experiments is becoming rampantly utilized in order to reduce the ...
Tarek Elsayed, Rania M. Hathout
doaj   +1 more source

Ultrasound-assisted extraction of polyphenols from Phyllanthi Fructus: Comprehensive insights from extraction optimization and antioxidant activity

Ultrasonics Sonochemistry
Phyllanthi Fructus (PF) is a valuable botanical resource with a long history of traditional use, known for potent antioxidant and anti-inflammatory effects attributed to its rich contents of bioactive compounds, particularly polyphenols. However, current
Haixia Che, Rui Zhang, Xianyao Wang, Hong Yu, Xuepeng Shi, Jie Yi, Jiawen Li, Qi Qi, Ruizhi Dong, Qian Li   +9 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Tuning structural dynamics through architected inner material designs: Numerical, experimental, and machine learning analysis

Materials & Design
Architected materials and structures have garnered significant interest due to their potential to furnish mechanical performances beyond the bounds of customary designs.
Irfan Ul Hassan, Agyapal Singh, Dimitrios C. Rodopoulos, Alexander Morawietz, Andrea Bergamini, Nikolaos Karathanasopoulos   +5 more
doaj   +1 more source