Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
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How to Unmask an Unknown: The Restriction-Modification System <i>Mho</i>VII of <i>Mycoplasma hominis</i> Expresses Two Complementary Methylation Activities in One Enzyme. [PDF]
Int J Mol SciVogelgsang L +5 more
europepmc +3 more sources
Multiple restrictionâmodification systems are present in rumen treponemes [PDF]
FEMS Microbiology Letters, 2005 Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases.
Maria, Piknova +2 more
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Phase variable restrictionâmodification systems inMoraxella catarrhalis [PDF]
FEMS Immunology & Medical Microbiology, 2002 A repetitive DNA motif was used as a marker to identify novel genes in the mucosal pathogen Moraxella catarrhalis. There is a high prevalence of such repetitive motifs in virulence genes that display phase variable expression. Two repeat containing loci were identified using a digoxigenin-labelled 5'-(CAAC)6-3' oligonucleotide probe.
Seib, K. L. +2 more
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IS-linked movement of a restriction-modification system. [PDF]
PLoS ONE, 2011 Potential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory ...
Noriko Takahashi +4 more
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A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus. [PDF]
PLoS One, 2022 Swinnen S +7 more
europepmc +3 more sources
Characterization of the NgoAXP: phase-variable type III restrictionâmodification system inNeisseria gonorrhoeae [PDF]
FEMS Microbiology Letters, 2009 Methyltransferases associated with type III restriction-modification (RM) systems are phase-variably expressed in a variety of pathogenic bacteria. NgoAXP, the type III RM system encoded by Neisseria gonorrhoeae, was characterized in this study. The cloned resngoAXP and ngoAXPmod genes were expressed in Escherichia coli strains.
Monika, Adamczyk-Poplawska +2 more
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A novel restrictionâmodification system fromXanthomonas campestrispv. vesicatoria encodes a m4C-methyltransferase and a nonfunctional restriction endonuclease [PDF]
FEMS Microbiology Letters, 2007 A novel restriction-modification (R-M) system, designated as xveIIRM, from chromosomal DNA of the Xanthomonas campestris pv. vesicatoria strain 7-1 (Xcv7-1) was cloned and characterized. The xveIIRM genes involved in this R-M system are aligned in a tail-to-tail orientation and overlapped by 12 base pairs.
Yu-Jen, Yu, Ming-Te, Yang
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Complete genome assembly and methylome dissection of Methanococcus aeolicus PL15/Hp
Frontiers in Microbiology, 2023 Although restriction-modification systems are found in both Eubacterial and Archaeal kingdoms, comparatively less is known about patterns of DNA methylation and genome defense systems in archaea.
Alexey Fomenkov +4 more
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PLoS Genetics, 2022 Understanding mechanisms that shape horizontal exchange in prokaryotes is a key problem in biology. A major limit on DNA entry is imposed by restriction-modification (RM) processes that depend on the pattern of DNA modification at host-specified sites ...
Julie Zaworski +6 more
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