Microbiology Spectrum, 2023 Myriad bacterial anti-phage systems have been described and often the mechanism of programmed cell death is invoked for phage inhibition. However, there is little evidence of “suicide” under physiological conditions for these systems. Instead of death to
Laura Fernández-García +6 more
doaj +2 more sources
Controller protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock. [PDF]
Nucleic Acids Res, 2018 C-proteins control restriction–modification (R–M) systems’ genes transcription to ensure sufficient levels of restriction endonuclease to allow protection from foreign DNA while avoiding its modification by excess methyltransferase. Here, we characterize
Klimuk E +7 more
europepmc +2 more sources
A Type III restriction–modification system in Mycoplasma mycoides subsp. capri [PDF]
Open Biology, 2012 The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction–modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and ...
Mikkel A. Algire +4 more
doaj +2 more sources
Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve. [PDF]
Nucleic Acids Res, 2018 Bifidobacterium breve represents one of the most abundant bifidobacterial species in the gastro-intestinal tract of breast-fed infants, where their presence is believed to exert beneficial effects.
Bottacini F +10 more
europepmc +2 more sources
Type II restriction modification system in Ureaplasma parvum OMC-P162 strain. [PDF]
PLoS ONE, 2018 Ureaplasma parvum serovar 3 strain, OMC-P162, was isolated from the human placenta of a preterm delivery at 26 weeks' gestation. In this study, we sequenced the complete genome of OMC-P162 and compared it with other serovar 3 strains isolated from ...
Heng Ning Wu +8 more
doaj +2 more sources
hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Survival Ability in Phagocytes. [PDF]
Front Microbiol, 2017 Streptococcus suis serotype 2 (SS2) is an important zoonotic agent in swine and humans. Anti-phagocytosis and survival in phagocytic cells and whole blood is essential for bacteria to be pathogenic.
Xu B +5 more
europepmc +2 more sources
From sequence to activity: the HgaI-homologous restriction modification system RM.MhoVI of Mycoplasma hominis [PDF]
BMC MicrobiologyRestriction-modification (RM) systems are widespread defense mechanisms in prokaryotes that protect the host from potentially harmful foreign DNA. They typically consist of a DNA methyltransferase (MTase), which methylates the host genome at an adenine ...
Lars Vogelgsang +5 more
doaj +2 more sources
Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system. [PDF]
Nucleic Acids Res, 2016 Type II restriction-modification (R-M) systems encode a restriction endonuclease that cleaves DNA at specific sites, and a methyltransferase that modifies same sites protecting them from restriction endonuclease cleavage.
Morozova N +9 more
europepmc +2 more sources
An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system [PDF]
Heliyon, 2019 Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova +3 more
doaj +2 more sources
Natural C-independent expression of restriction endonuclease in a C protein-associated restriction-modification system. [PDF]
Nucleic Acids Res, 2016 Restriction–modification (R-M) systems are highly prevalent among bacteria and archaea, and appear to play crucial roles in modulating horizontal gene transfer and protection against phage.
Rezulak M, Borsuk I, Mruk I.
europepmc +2 more sources