Results 51 to 60 of about 605,403 (299)

Analysis of the subunit assembly of the type IC restriction-modification enzyme EcoR124I [PDF]

, 1998
Type I restriction-modification (R-M) enzymes are composed of three different subunits, of which HsdS determines DNA specificity, HsdM is responsible for DNA methylation and HsdR is required for restriction.
Dryden, D T F, Firman, K, Janscak, P
core   +2 more sources

Transcription regulation of restriction-modification system Ecl18kI [PDF]

Nucleic Acids Research, 2009
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription is achieved through a separate DNA-binding domain of the methyltransferase. M.Ecl18kI recognizes an operator sequence located in the noncoding region that separates the divergently transcribed R and M genes.
Protsenko, Alexey, Zakharova, Marina, Nagornykh, Maxim, Solonin, Alexander, Severinov, Konstantin   +4 more
openaire   +2 more sources

Transcription regulation of restriction-modification system Esp1396I [PDF]

Nucleic Acids Research, 2009
Abstract The convergently transcribed restriction (R) and methylase (M) genes of the Restriction–Modification system Esp1396I are tightly regulated by a controller (C) protein that forms part of the CR operon. We have mapped the transcriptional start sites from each promoter and examined the regulatory role of C.Esp1396I in vivo and in ...
Bogdanova, E., Zakharova, M., Streeter, Simon, Taylor, J., Heyduk, T., Kneale, Geoff, Severinov, K.   +6 more
openaire   +3 more sources

Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]

, 2015
Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas, LaFayette, Peter R, Parrott, Wayne A, Schmitz, Robert J   +3 more
core   +2 more sources

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences [PDF]

, 2013
Bacterial phosphorothioate (PT) DNA modifications are incorporated by Dnd proteins A-E and often function with DndF-H as a restriction-modification (R-M) system, as in Escherichia coli B7A. However, bacteria such as Vibrio cyclitrophicus FF75 lack dndF-H,
Boitano, Matthew, Butty, Vincent, Cao, Bo, Chen, Chao, Chen, Shi, Cheng, Qiuxiang, Clark, Tyson A., Dedon, Peter C., DeMott, Michael S., Deng, Zixin, Korlach, Jonas, Levine, Stuart S., Luong, Khai, Song, Yi, Turner, Stephen W., Wang, Lianrong, Xiong, Xiaolin, You, Delin, Yuan, George, Zheng, Xiaoqing, Zhou, Xiufen   +20 more
core   +1 more source

Organization of restriction-modification systems

Nucleic Acids Research, 1991
The genes for over 100 restriction-modification systems have now been cloned, and approximately one-half have been sequenced. Despite their similar function, they are exceedingly heterogeneous. The heterogeneity is evident at three levels: in the gene arrangements; in the enzyme compositions; and in the protein sequences. This paper summarizes the main
openaire   +3 more sources

Is General Relativity a simpler theory? [PDF]

, 2012
Gravity is understood as a geometrization of spacetime. But spacetime is also the manifold of the boundary values of the spinless point particle in a variational approach. Since all known matter, baryons, leptons and gauge bosons are spinning objects, it
Martín Rivas, Rivas M, Rivas M, Rund H
core   +2 more sources

To be or not to be: regulation of restriction–modification systems and other toxin–antitoxin systems [PDF]

Nucleic Acids Research, 2013
One of the simplest classes of genes involved in programmed death is that containing the toxin-antitoxin (TA) systems of prokaryotes. These systems are composed of an intracellular toxin and an antitoxin that neutralizes its effect. These systems, now classified into five types, were initially discovered because some of them allow the stable ...
Mruk, Iwona, Kobayashi, Ichizo
openaire   +2 more sources

Avoidance of recognition sites of restriction-modification systems is a widespread but not universal anti-restriction strategy of prokaryotic viruses

BMC Genomics, 2018
Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov, A. S. Ershova, A. S. Karyagina, S. A. Spirin, A. V. Alexeevski   +4 more
doaj   +1 more source

Effect of minimal length uncertainty on the mass-radius relation of white dwarfs

, 2017
Generalized uncertainty relation that carries the imprint of quantum gravity introduces a minimal length scale into the description of space-time. It effectively changes the invariant measure of the phase space through a factor $(1+\beta \mathbf{p}^2 ...
Mathew, Arun, Nandy, Malay K.
core   +1 more source