Cloning of Mannose-1-Phosphate Guanyltransferase Encoding Gene (MRHO/IR/ER/75) in Leishmania Major
Background: Leishmania is an obligate interacellular protozoa and sand fly, as a vector, transmits infectious forms of the parasite to vertebrate host.
Noosha Zia +6 more
doaj
Genetic recombination without using either restriction enzyme or PCR [PDF]
Jing-Min Zhou +3 more
openalex +1 more source
Here, we introduced an intermittent electrical stimulation protocol mimicking the episodic nature of real‐life exercise in vitro by alternating low‐ and high‐frequency stimulation. In comparison with widely used continuous stimulation, it enhanced the rate of glucose and fatty acid oxidation, but not the myokine release.
Klára Gabrišová +11 more
wiley +1 more source
Berberine–cinnamic acid co‐crystal (BBR‐CA) inhibits the phosphorylation of the phosphatidylinositol 3‐kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway, suppressing the transfer of pre‐sterol regulatory element‐binding proteins‐1 (SREBP‐1) from the endoplasmic reticulum to the nucleus. This results in a decrease in the expression level of
Wenheng Gao +7 more
wiley +1 more source
Biochemical and mutational analysis of EcoRII functional domains reveals evolutionary links between restriction enzymes [PDF]
Gintautas Tamulaitis +2 more
openalex +1 more source
Controlling the enzymatic activity of a restriction enzyme by light
B. Schierling +10 more
semanticscholar +1 more source
Epigallocatechin‐3‐gallate (EGCG) acutely inhibited gluconeogenesis and enhanced glycolysis, glycogenolysis, and fatty acid oxidation in perfused rat livers. Mechanistic assays revealed mitochondrial uncoupling, inhibition of pyruvate carboxylation and glucose‐6‐phosphatase, shift of NADH/NAD+ ratios toward oxidation, and loss of membrane integrity ...
Carla Indianara Bonetti +8 more
wiley +1 more source
KasI vs. SspDI: Comparative analysis of enzyme activity for restriction digestion [PDF]
Suman, Rekha Puria, Vikrant Nain
openalex
Characterization of Biomphalaria orbignyi, Biomphalaria peregrina and Biomphalaria oligoza by polymerase chain reaction and restriction enzyme digestion of the internal transcribed spacer region of the RNA ribosomal gene [PDF]
Linus Spatz +4 more
openalex +1 more source
Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini +2 more
wiley +1 more source

