Results 221 to 230 of about 647,244 (359)
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada+3 more
wiley +1 more source
I-PREFR: Inverse PCR-Based Restriction Enzyme FRee Unidirectional Strategy for Rapid Markerless Chromosomal Gene Deletion and Reconstitution in Bacteria Using Suicide Vectors. [PDF]
Rana R, Sharma A, Dutta A, Patil PB.
europepmc +1 more source
Molecular Cloning and Restriction Enzyme Mapping of an African Swine Fever Virus Isolate from Malawi
Linda K. Dixon
openalex +1 more source
Detection of elsamicin‐DNA binding specificity by restriction enzyme cleavage [PDF]
A. Parraga, José Portugal
openalex +1 more source
Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa+2 more
wiley +1 more source
A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure. [PDF]
Fitzgerald S, Bhat B, Print C, Jones GT.
europepmc +1 more source
II-Q restriction endonucleases—new class of type II enzymes [PDF]
S. Kh. Degtyarev+4 more
openalex +1 more source
Aging weakens the blood–brain barrier (BBB), increasing susceptibility to CNS cancers and complicating treatment. This review examines BBB deterioration, its impact on drug delivery, and potential interventions like targeting neuroinflammation and advanced therapies.
Quang La, Aiman Baloch, David F. Lo
wiley +1 more source
Tumor diversity and evolution revealed through RADseq [PDF]
Altshuler+66 more
core +2 more sources