Results 21 to 30 of about 347,445 (313)

"A PCR-RFLP Method to Identification of the Important Opportunistic Fungi: Candida Species, Cryptococcus neoformans, Aspergillus famigatus and Fusarium solani" [PDF]

open access: yesIranian Journal of Public Health, 2001
Deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. DNA-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy.
SH Mirhendi   +5 more
doaj   +1 more source

Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes

open access: yes한국균학회지, 2020
Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other ...
Mi-Jeong Park   +3 more
doaj   +1 more source

Restricting SBH Ambiguity via Restriction Enzymes

open access: yesDiscrete Applied Mathematics, 2002
AbstractSequencing by hybridization (SBH) is a proposed approach to DNA sequencing. The SBH-spectrum of the target sequence is a list of all k-mers occurring at least once in the sequence. Sequencing is successful if the SBH-spectrum is a result of only that sequence and ambiguous otherwise.
Steven Skiena, Sagi Snir
openaire   +2 more sources

Type I restriction enzymes and their relatives [PDF]

open access: yesNucleic Acids Research, 2013
Type I restriction enzymes (REases) are large pentameric proteins with separate restriction (R), methylation (M) and DNA sequence-recognition (S) subunits. They were the first REases to be discovered and purified, but unlike the enormously useful Type II REases, they have yet to find a place in the enzymatic toolbox of molecular biologists.
Loenen, Wil A M   +3 more
openaire   +4 more sources

Restriction Enzyme Digestion Exercise – An In-class Activity

open access: yesJournal of Microbiology & Biology Education, 2010
Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends
Michelle Parent
doaj   +1 more source

REBASE--restriction enzymes and DNA methyltransferases [PDF]

open access: yesNucleic Acids Research, 2004
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Roberts, Richard J.   +3 more
openaire   +2 more sources

REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance

open access: yesBMC Bioinformatics, 2006
Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E   +2 more
doaj   +1 more source

Methoden zur Untersuchung der Allelfrequenzen und-verteilung im Acetolactat-Synthase (ALS)-Gen bei Target-Site-Resistenzen in Echinochloa crus-galli

open access: yesJulius-Kühn-Archiv, 2020
Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian   +4 more
doaj   +1 more source

Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector

open access: yesViruses, 2022
Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field.
Xiaojuan Guo   +7 more
doaj   +1 more source

Nucleoside triphosphate-dependent restriction enzymes [PDF]

open access: yesNucleic Acids Research, 2001
The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
Dryden, DTF, Murray, Noreen E, Rao, DN
openaire   +4 more sources

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