Results 21 to 30 of about 607,747 (257)
Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities [PDF]
, 2015 The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in ...
Fukuyo, Masaki +9 more
core +2 more sources
Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving. [PDF]
, 2011 Background The TaqII enzyme is a member of the Thermus sp. enzyme family that we propounded previously within Type IIS restriction endonucleases, containing related thermophilic bifunctional endonucleases-methyltransferases from various ...
Agnieszka Zylicz-Stachula +4 more
core +1 more source
"A PCR-RFLP Method to Identification of the Important Opportunistic Fungi: Candida Species, Cryptococcus neoformans, Aspergillus famigatus and Fusarium solani" [PDF]
Iranian Journal of Public Health, 2001 Deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. DNA-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy.
SH Mirhendi +5 more
doaj +1 more source
Restriction Enzyme Digestion Exercise – An In-class Activity
Journal of Microbiology & Biology Education, 2010 Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends
Michelle Parent
doaj +1 more source
Julius-Kühn-Archiv, 2020 Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian +4 more
doaj +1 more source
Staphylococcus aureus typing by digestion of protein A coding gene using Bsp143I [PDF]
, 2014 Background: Protein A is the virulence factors of Staphylococcus aureus rolling in its pathogenesis, and its gene is used for typing. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with different enzymes has been used for ...
Ghaemi, E.A. +2 more
core +1 more source
RestrictionDigest: A powerful Perl module for simulating genomic restriction digests
Electronic Journal of Biotechnology, 2016 Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang +4 more
doaj +1 more source
한국균학회지, 2020 Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other ...
Mi-Jeong Park +3 more
doaj +1 more source
DNA Cloning without Restriction Enzyme and Ligase
BioTechniques, 1999 One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
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Exploration of MedicineStreptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on ...
Marina Yusoff +3 more
doaj +1 more source