Results 21 to 30 of about 3,197,803 (398)

A method for identifying allele-specific hydroxymethylation

Epigenetics, 2020
We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
doaj   +1 more source

Kinetics of target site localization of a protein on DNA: a stochastic approach [PDF]

, 2004
It is widely recognized that the cleaving rate of a restriction enzyme on target DNA sequences is several orders of magnitude faster than the maximal one calculated from the diffusion--limited theory.
Berg, Berg, Berg, Berg, Erskine, Halford, Jeltsch, Langowski, Lohman, Milsom, Misteli, Redner, Richter, Riggs, Shimamoto, Stanford, Szczelkun, Taylor, Terry, Von Hippel, Wenner, Winter   +21 more
core   +3 more sources

Restriction enzymes and their isoschizomers [PDF]

Nucleic Acids Research, 1987
Since the last compilation of restriction enzymes (1), 300 new entries have been added including 12 new specificities. With the growing size of this database and the recognition that the most widespread use of the information is as a database for computer programs predicting restriction enzyme cleavage patterns, the new format has been continued ...
openaire   +12 more sources

REBASE: restriction enzymes and methyltransferases [PDF]

Nucleic Acids Research, 2003
REBASE contains comprehensive information about restriction enzymes, DNA methyltransferases and related proteins such as nicking enzymes, specificity subunits and control proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data. Homing endonucleases are
Tamas Vincze, Richard J. Roberts, Janos Posfai, Dana Macelis   +3 more
openaire   +3 more sources

Optimized In Vitro Restriction Digestion Protocol for Preparing Maize and Barley ddRAD-Seq Libraries

Agronomy, 2023
In recent years, high-throughput sequencing methods have become increasingly popular in molecular biology laboratories, mainly due to the relatively low cost of small, benchtop platforms, the simplicity of library preparation, and the low price per unit ...
Marta Puchta-Jasińska, Paulina Bolc, Urszula Piechota, Maja Boczkowska   +3 more
doaj   +1 more source

Cleavage of a model DNA replication fork by a Type I restriction endonuclease [PDF]

, 2009
Cleavage of a DNA replication fork leads to fork restoration by recombination repair. In prokaryote cells carrying restriction–modification systems, fork passage reduces genome methylation by the modification enzyme and exposes the chromosome to attack ...
Agarkova, Aravind, Arifuzzaman, Bianco, Bickle, Blakely, Bremer, Ciccia, Ciccia, Cox, Cromie, Davies, Day, Doronina, Dreier, Dunderdale, Efimova, Ellis, Eykelenboom, Firman, Froget, Garcia, Gari, Gari, Handa, Heller, Hochegger, Hong, Ichizo Kobayashi, Ip, Ivancic-Bacce, Iwasaki, Janscak, Janscak, Janscak, Ken Ishikawa, Kobayashi, Kogoma, Komori, Komori, Kreuzer, Kusano, Kusano, Long, Makovets, Makovets, McClelland, McClelland, McGlynn, McGlynn, Meetei, Mernagh, Michel, Mosedale, Murray, Naito, Naofumi Handa, Nishino, Osman, Pages, Panyutin, Pingoud, Prakash, Price, Roos, Seidel, Seidel, Seigneur, Stanley, Stanley, Studier, Sun, Szczelkun, Takahashi, Taylor, Wang, Weinstock, Yuan   +77 more
core   +2 more sources

The Architecture of Restriction Enzymes [PDF]

Structure, 2013
In this issue of Structure, Lyumkis and colleagues describe a high resolution structure of a polymerized form of the SgrAI restriction enzyme, which shows that it forms a helical assembly with four enzyme molecules per turn of the helix. The DNA is arranged on the periphery of the protein helix pointing away from the helical axis.
openaire   +3 more sources

Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities [PDF]

, 2015
The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in ...
Fukuyo, Masaki, Furuta, Yoshikazu, Hamakawa, Takeshi, Ide, Hiroshi, Ishikawa, Ken, Kobayashi, Ichizo, Nakano, Toshiaki, Watanabe-Matsui, Miki, Yano, Hirokazu, Zhang, Yingbiao   +9 more
core   +2 more sources

Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis

Journal of Clinical Microbiology, 1993
A method for the rapid identification of mycobacteria to the species level was developed on the basis of evaluation by the polymerase chain reaction (PCR) of the gene encoding for the 65-kDa protein. The method involves restriction enzyme analysis of PCR
A. Telenti, F. Marchesi, Marianne Balz, Frank, Bally, Erik C. BOTrGER, T. Bodmer   +6 more
semanticscholar   +1 more source

Sequence organization of feline leukemis virus DNA in infected cells [PDF]

, 1980
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W., Davidson, Norman, Mullins, James I., Nicolson, Margery O.   +3 more
core   +1 more source