Results 21 to 30 of about 2,983,763 (344)

The Architecture of Restriction Enzymes [PDF]

Structure, 2013
In this issue of Structure, Lyumkis and colleagues describe a high resolution structure of a polymerized form of the SgrAI restriction enzyme, which shows that it forms a helical assembly with four enzyme molecules per turn of the helix. The DNA is arranged on the periphery of the protein helix pointing away from the helical axis.
openaire   +2 more sources

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries

BioTechniques, 2016
Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang, Wayne A. Johnston, Mikael Boden, Elizabeth M.J. Gillam   +3 more
doaj   +1 more source

A method for identifying allele-specific hydroxymethylation

Epigenetics, 2020
We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
doaj   +1 more source

"A PCR-RFLP Method to Identification of the Important Opportunistic Fungi: Candida Species, Cryptococcus neoformans, Aspergillus famigatus and Fusarium solani" [PDF]

Iranian Journal of Public Health, 2001
Deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. DNA-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy.
SH Mirhendi, P Kordbacheh, B Kazemi, S Samiei, M Pezeshki, MR Khorramizadeh   +5 more
doaj   +1 more source

Genomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP

Frontiers in Microbiology, 2016
The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry.
D. Mandakovic, B. Glasner, J. Maldonado, P. Aravena, Mauricio González, V. Cambiazo, R. Pulgar   +6 more
semanticscholar   +1 more source

Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI

Brazilian Journal of Microbiology, 2014
In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically important Aspergillus species.
K. Diba, Hossein Mirhendi, P. Kordbacheh, S. Rezaie   +3 more
semanticscholar   +1 more source

Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods

Annual International Conference on Research in Computational Molecular Biology, 2013
Recent advancements in sequencing-based DNA methylation profiling methods provide an unprecedented opportunity to map complete DNA methylomes. These include whole-genome bisulfite sequencing (WGBS, MethylC-seq, or BS-seq), reduced-representation ...
Michael Stevens, Jeffrey B. Cheng, Mingchao Xie, J. Costello, Ting Wang   +4 more
semanticscholar   +1 more source

Double restriction-enzyme digestion improves the coverage and accuracy of genome-wide CpG methylation profiling by reduced representation bisulfite sequencing

BMC Genomics, 2013
BackgroundReduced representation bisulfite sequencing (RRBS) was developed to measure DNA methylation of high-CG regions at single base-pair resolution, and has been widely used because of its minimal DNA requirements and cost efficacy; however, the CpG ...
Junwen Wang, Y. Xia, Lili Li, Desheng Gong, Yu Yao, Huijuan Luo, Hanlin Lu, Na Yi, Honglong Wu, Xiuqing Zhang, Q. Tao, F. Gao   +11 more
semanticscholar   +1 more source

Methylcytosine and Normal Cytosine Deamination by the Foreign DNA Restriction Enzyme APOBEC3A*

Journal of Biological Chemistry, 2012
Background: APOBEC3A is a myeloid-specific interferon-inducible DNA C to U deaminase implicated in innate immunity. Results: APOBEC3A also elicits MeC to T editing activity in vitro with deoxy-oligonucleotides and in vivo with transfected plasmids ...
M. Carpenter, Ming Li, Anurag Rathore, Lela Lackey, Emily K Law, Allison M. Land, B. Leonard, S. Shandilya, M. Bohn, C. Schiffer, W. Brown, R. Harris   +11 more
semanticscholar   +1 more source

Methoden zur Untersuchung der Allelfrequenzen und-verteilung im Acetolactat-Synthase (ALS)-Gen bei Target-Site-Resistenzen in Echinochloa crus-galli

Julius-Kühn-Archiv, 2020
Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian, Keil, Sven, Löbmann, Anja, Petersen, Jan, Brändle, Frank   +4 more
doaj   +1 more source