Results 31 to 40 of about 612,357 (298)

Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes

open access: yes한국균학회지, 2020
Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other ...
Mi-Jeong Park   +3 more
doaj   +1 more source

DNA Cloning without Restriction Enzyme and Ligase

open access: yesBioTechniques, 1999
One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
doaj   +1 more source

Staphylococcus aureus typing by digestion of protein A coding gene using Bsp143I [PDF]

open access: yes, 2014
Background: Protein A is the virulence factors of Staphylococcus aureus rolling in its pathogenesis, and its gene is used for typing. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with different enzymes has been used for ...
Ghaemi, E.A.   +2 more
core   +1 more source

Endonuclease A in Streptococcus pneumoniae: escaping from neutrophil extracellular traps (NETs) and relationship in immunogenicity

open access: yesExploration of Medicine
Streptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on ...
Marina Yusoff   +3 more
doaj   +1 more source

Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP

open access: yesJournal of Biological Researches, 2012
This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro   +3 more
doaj   +1 more source

The baboon endogenous virus genome. II. Provirus sequence variations in baboon cell DNA [PDF]

open access: yes, 1980
Restriction analysis of the approximately 100 integrated baboon endogenous virus (BaEV) proviruses in baboon cells and tissues has revealed two major sequence variations, both in the gag gene region of the genome.
Cohen, Maurice   +5 more
core   +1 more source

Computer Aided Simulation of DNA Fingerprint Amplified Fragment Length Polymophism (AFLP) Using Suffix Tree Indexing and Data Mining [PDF]

open access: yes, 2011
AFLP is one of the DNA Fingerprinting techniques which have broad application as genetic marker in various fields. Begin with the DNA sequence digestion using one or more particular restriction enzyme, ligation of the adapters to the overhanging sticky ...
Budiman, Agung   +3 more
core  

A solution to the subdiffusion-efficiency paradox: Inactive states enhance reaction efficiency at subdiffusion conditions in living cells

open access: yes, 2012
Macromolecular crowding in living biological cells effects subdiffusion of larger biomolecules such as proteins and enzymes. Mimicking this subdiffusion in terms of random walks on a critical percolation cluster, we here present a case study of EcoRV ...
Lomholt, Michael A.   +2 more
core   +1 more source

Cloning and characterisation of chlorophyll synthase from Avena sativa [PDF]

open access: yes, 2001
The chlorophyll synthase gene from oat (Avena sativa) was cloned and expressed in Escherichia coli. The deduced amino acid sequence consists of 378 amino acids including a presequence, of 46 amino acids.
Kögel, Julia   +4 more
core   +1 more source

Personalized Selumetinib Dosing in Pediatric Neurofibromatosis Type 1: Insights From a Pilot Therapeutic Drug Monitoring Study

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Objective To evaluate selumetinib exposure using therapeutic drug monitoring (TDM) in pediatric patients with neurofibromatosis type 1 (NF1) and plexiform neurofibromas (PN), assess interpatient pharmacokinetic variability, and explore the relationship between drug exposure, clinical response, and adverse effects.
Janka Kovács   +8 more
wiley   +1 more source

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