Results 21 to 30 of about 612,357 (298)

Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao

Brazilian Archives of Biology and Technology, 2008
The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the
Francis Julio Fagundes Lopes, Marisa Vieira de Queiroz, Juliana Oliveira Lima, Viviane Aline Oliveira Silva, Elza Fernandes de Araújo   +4 more
doaj   +1 more source

Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat

Tropical Animal Science Journal, 2023
Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik, F. A. Z. Chairunissa, S. Bintara, F. J. Fadilllah, N. P. Ningrum, D. Puspitasari, Kustantinah   +6 more
doaj   +1 more source

Lack of class I H-2 antigens in cells transformed by radiation leukemia virus is associated with methylation and rearrangement of H-2 DNA [PDF]

, 1986
Transformation of murine thymocytes by radiation leukemia virus is associated with reduced expression of the class I antigens encoded in the major histocompatibility complex (MHC) and increased methylation and altered restriction enzyme patterns of MHC ...
Boral, Anthony, Buxbaum, Joel, Devlin, J., Flavell, Richard A., Kornreich, Ruth, Mellor, Andrew L., Meruelo, Daniel, Pampeno, Christine, Pellicer, Angel, Rossomando, Anthony, Silver, Jack L., Weiss, Elisabeth H.   +11 more
core   +1 more source

Trisomy 21 alters DNA methylation in parent-of-origin-dependent and independent manners [PDF]

, 2016
The supernumerary chromosome 21 in Down syndrome differentially affects the methylation statuses at CpG dinucleotide sites and creates genome-wide transcriptional dysregulation of parental alleles, ultimately causing diverse pathologies.
Alves da Silva, Antônio Francisco, Biselli-Périco, Joice Matos, Chuva de Sousa Lopes, Susana Marina, Fernandes, Maria Gomes, Francisco Junior, Ronaldo da Silva, Lopes Rios, Álvaro Fabricio, Machado, Douglas Terra, Machado, Filipe Brum, Medina-Acosta, Enrique, Mozer Rodrigues, Pedro Thyago, Pavarino, Érika Cristina, Santos-Rebouças, Cíntia Barros, Zampieri, Bruna Lancia   +12 more
core   +7 more sources

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries

BioTechniques, 2016
Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang, Wayne A. Johnston, Mikael Boden, Elizabeth M.J. Gillam   +3 more
doaj   +1 more source

Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving. [PDF]

, 2011
Background The TaqII enzyme is a member of the Thermus sp. enzyme family that we propounded previously within Type IIS restriction endonucleases, containing related thermophilic bifunctional endonucleases-methyltransferases from various ...
Agnieszka Zylicz-Stachula, Olga Zolnierkiewicz, Katarzyna Sliwinska, Joanna Jezewska-Frackowiak, Piotr Skowron   +4 more
core   +1 more source

"A PCR-RFLP Method to Identification of the Important Opportunistic Fungi: Candida Species, Cryptococcus neoformans, Aspergillus famigatus and Fusarium solani" [PDF]

Iranian Journal of Public Health, 2001
Deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. DNA-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy.
SH Mirhendi, P Kordbacheh, B Kazemi, S Samiei, M Pezeshki, MR Khorramizadeh   +5 more
doaj   +1 more source

Restriction Enzyme Digestion Exercise – An In-class Activity

Journal of Microbiology & Biology Education, 2010
Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends
Michelle Parent
doaj   +1 more source

Methoden zur Untersuchung der Allelfrequenzen und-verteilung im Acetolactat-Synthase (ALS)-Gen bei Target-Site-Resistenzen in Echinochloa crus-galli

Julius-Kühn-Archiv, 2020
Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian, Keil, Sven, Löbmann, Anja, Petersen, Jan, Brändle, Frank   +4 more
doaj   +1 more source

RestrictionDigest: A powerful Perl module for simulating genomic restriction digests

Electronic Journal of Biotechnology, 2016
Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang, Li Li, Haigang Qi, Xuedi Du, Guofan Zhang   +4 more
doaj   +1 more source