Results 31 to 40 of about 2,983,763 (344)
Scientific Papers Animal Science and Biotechnologies, 2023 BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic
Viorica Coşier
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Restriction Enzyme Digestion Exercise – An In-class Activity
Journal of Microbiology & Biology Education, 2010 Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends
Michelle Parent
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Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector
Viruses, 2022 Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field.
Xiaojuan Guo +7 more
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Nucleic Acids Research, 2011 A gene encoding a putative DNA helicase from Staphylococcus aureus USA300 was cloned and expressed in Escherichia coli. The protein was purified to over 90% purity by chromatography.
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BMC Bioinformatics, 2006 Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E +2 more
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한국균학회지, 2020 Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other ...
Mi-Jeong Park +3 more
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Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat
Tropical Animal Science Journal, 2023 Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik +6 more
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RestrictionDigest: A powerful Perl module for simulating genomic restriction digests
Electronic Journal of Biotechnology, 2016 Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang +4 more
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Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)
PeerJ, 2019 Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing
N. Bayona‐Vásquez +12 more
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DNA Cloning without Restriction Enzyme and Ligase
BioTechniques, 1999 One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
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