Results 31 to 40 of about 3,197,803 (398)

Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI

Brazilian Journal of Microbiology, 2014
In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically important Aspergillus species.
K. Diba, Hossein Mirhendi, P. Kordbacheh, S. Rezaie   +3 more
semanticscholar   +1 more source

Restriction Enzyme Recognition Sequence Search Program

BioTechniques, 2002
A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search ...
Kyle P. Ellrott, Julie K.A. Kasarjian, Tao Jiang, Junichi Ryu   +3 more
doaj   +1 more source

Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods

Annual International Conference on Research in Computational Molecular Biology, 2013
Recent advancements in sequencing-based DNA methylation profiling methods provide an unprecedented opportunity to map complete DNA methylomes. These include whole-genome bisulfite sequencing (WGBS, MethylC-seq, or BS-seq), reduced-representation ...
Michael Stevens, Jeffrey B. Cheng, Mingchao Xie, J. Costello, Ting Wang   +4 more
semanticscholar   +1 more source

Restriction Enzyme Digestion Exercise – An In-class Activity

Journal of Microbiology & Biology Education, 2010
Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends
Michelle Parent
doaj   +1 more source

Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat

Tropical Animal Science Journal, 2023
Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik, F. A. Z. Chairunissa, S. Bintara, F. J. Fadilllah, N. P. Ningrum, D. Puspitasari, Kustantinah   +6 more
doaj   +1 more source

Lack of class I H-2 antigens in cells transformed by radiation leukemia virus is associated with methylation and rearrangement of H-2 DNA [PDF]

, 1986
Transformation of murine thymocytes by radiation leukemia virus is associated with reduced expression of the class I antigens encoded in the major histocompatibility complex (MHC) and increased methylation and altered restriction enzyme patterns of MHC ...
Boral, Anthony, Buxbaum, Joel, Devlin, J., Flavell, Richard A., Kornreich, Ruth, Mellor, Andrew L., Meruelo, Daniel, Pampeno, Christine, Pellicer, Angel, Rossomando, Anthony, Silver, Jack L., Weiss, Elisabeth H.   +11 more
core   +1 more source

Enzyme kinetics at the molecular level [PDF]

Resonance - Journal of Science Education, 24(3), 297 (2019), 2019
The celebrated Michaelis-Menten (MM) expression provides a fundamental relation between the rate of enzyme catalysis and substrate concentration. The validity of this classical expression is, however, restricted to macroscopic amounts of enzymes and substrates and, thus, to processes with negligible fluctuations.
arxiv   +1 more source

Double restriction-enzyme digestion improves the coverage and accuracy of genome-wide CpG methylation profiling by reduced representation bisulfite sequencing

BMC Genomics, 2013
BackgroundReduced representation bisulfite sequencing (RRBS) was developed to measure DNA methylation of high-CG regions at single base-pair resolution, and has been widely used because of its minimal DNA requirements and cost efficacy; however, the CpG ...
Junwen Wang, Y. Xia, Lili Li, Desheng Gong, Yu Yao, Huijuan Luo, Hanlin Lu, Na Yi, Honglong Wu, Xiuqing Zhang, Q. Tao, F. Gao   +11 more
semanticscholar   +1 more source

A Sliding Restriction Enzyme Pauses [PDF]

Structure, 2007
In this issue of Structure, Aggarwal and colleagues (Townson et al., 2007) present the crystal structure of the restriction endonuclease BstYI in complex with a near-cognate substrate. This structure most likely reflects the conformation BstYI adopts as it scans DNA and pauses upon encountering a site similar to its recognition sequence.
Wolfgang Wende, Alfred Pingoud
openaire   +2 more sources

HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries)

Scientific Papers Animal Science and Biotechnologies, 2023
BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic
Viorica Coşier
doaj