Results 31 to 40 of about 620,807 (343)

REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance

BMC Bioinformatics, 2006
Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E, Martin Patrick, Pognonec Philippe   +2 more
doaj   +1 more source

Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes

한국균학회지, 2020
Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other ...
Mi-Jeong Park, Rhim Ryoo, Yeongseon Jang, Kang-Hyeon Ka   +3 more
doaj   +1 more source

Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities [PDF]

, 2015
The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in ...
Fukuyo, Masaki, Furuta, Yoshikazu, Hamakawa, Takeshi, Ide, Hiroshi, Ishikawa, Ken, Kobayashi, Ichizo, Nakano, Toshiaki, Watanabe-Matsui, Miki, Yano, Hirokazu, Zhang, Yingbiao   +9 more
core   +2 more sources

RestrictionDigest: A powerful Perl module for simulating genomic restriction digests

Electronic Journal of Biotechnology, 2016
Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang, Li Li, Haigang Qi, Xuedi Du, Guofan Zhang   +4 more
doaj   +1 more source

Staphylococcus aureus typing by digestion of protein A coding gene using Bsp143I [PDF]

, 2014
Background: Protein A is the virulence factors of Staphylococcus aureus rolling in its pathogenesis, and its gene is used for typing. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with different enzymes has been used for ...
Ghaemi, E.A., Kochkaksaraei, M.B., Shakeri, F.   +2 more
core   +1 more source

DNA Cloning without Restriction Enzyme and Ligase

BioTechniques, 1999
One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
doaj   +1 more source

2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador [PDF]

, 2017
Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru.
Andersson, Björn, Costales, Jaime A., De Noia, Michele, Grijalva, Mario J., Hernandez Castro, Luis Enrique, Hernandez-Castro, Luis E., Llewellyn, Martin S., Ocaña-Mayorga, Sofía, Paterno, Marta, Villacís, Anita G., Yumiseva, Cesar A.   +10 more
core   +4 more sources

Endonuclease A in Streptococcus pneumoniae: escaping from neutrophil extracellular traps (NETs) and relationship in immunogenicity

Exploration of Medicine
Streptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on ...
Marina Yusoff, Chew Chieng Yeo, Muhammad Hassan Nasir, Malik Amonov   +3 more
doaj   +1 more source

Natural polymorphism in the thrombospondin-related adhesive protein of Plasmodium falciparum [PDF]

, 1998
We have developed a typing system using natural sequence variation in the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium falciparum. This method permits a haplotype to be assigned to any particular TRAP gene.
Bosman, Andrea, Crisanti, Andrea, Dolo, Amagana, Doumbo, Ogobara, Hackford, Ian R., Keita, Mamadou M., Modiano, David, Richards, Martin B., Robson, Kathryn J.H., Sidibe, Touman   +9 more
core   +1 more source

Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP

Journal of Biological Researches, 2012
This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro, Sri Rahayu, T Susilawati, Soemarno Soemarno   +3 more
doaj   +1 more source