Results 31 to 40 of about 347,445 (313)

RestrictionDigest: A powerful Perl module for simulating genomic restriction digests

Electronic Journal of Biotechnology, 2016
Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang, Li Li, Haigang Qi, Xuedi Du, Guofan Zhang   +4 more
doaj   +1 more source

Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat

Tropical Animal Science Journal, 2023
Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik, F. A. Z. Chairunissa, S. Bintara, F. J. Fadilllah, N. P. Ningrum, D. Puspitasari, Kustantinah   +6 more
doaj   +1 more source

DNA Cloning without Restriction Enzyme and Ligase

BioTechniques, 1999
One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
doaj   +1 more source

Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP

Journal of Biological Researches, 2012
This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro, Sri Rahayu, T Susilawati, Soemarno Soemarno   +3 more
doaj   +1 more source

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

FEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

Genotyping and antimicrobial resistance patterns of Klebsiella pneumoniae clinical isolates in Suez Canal University Hospitals [PDF]

Microbes and Infectious Diseases
Background: Health crisis of multi-drug resistant Klebsiella pneumoniae (K. pneumoniae) seems overwhelming. Genotyping for such an important pathogen is a demand to control aggressive Klebsiella infections. Therefore, this study was conducted to identify
Maha Mahdi, Waheed Hessam, Atef Shehata, Marwa Fouad, Yara Marei   +4 more
doaj   +1 more source

Restriction enzyme digestion of hemimethylated DNA [PDF]

Nucleic Acids Research, 1981
Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar, Aharon Razin, Yosef Gruenbaum   +2 more
openaire   +3 more sources

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

Endonuclease A in Streptococcus pneumoniae: escaping from neutrophil extracellular traps (NETs) and relationship in immunogenicity

Exploration of Medicine
Streptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on ...
Marina Yusoff, Chew Chieng Yeo, Muhammad Hassan Nasir, Malik Amonov   +3 more
doaj   +1 more source