Results 31 to 40 of about 2,927,350 (287)

Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)

PeerJ, 2019
Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing
N. Bayona‐Vásquez, T. Glenn, Troy J. Kieran, Todd W. Pierson, Sandra L. Hoffberg, P. A. Scott, K. Bentley, J. W. Finger, Swarnali Louha, Nicholas J. Troendle, P. Díaz‐Jaimes, R. Mauricio, B. Faircloth   +12 more
semanticscholar   +1 more source

Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme Genotyping-by-Sequencing Approach

PLoS ONE, 2012
Advancements in next-generation sequencing technology have enabled whole genome re-sequencing in many species providing unprecedented discovery and characterization of molecular polymorphisms. There are limitations, however, to next-generation sequencing
J. Poland, P. Brown, M. Sorrells, J. Jannink   +3 more
semanticscholar   +1 more source

DNA Cloning without Restriction Enzyme and Ligase

BioTechniques, 1999
One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
doaj   +1 more source

Atomic force microscopy of the EcoKI Type I DNA restriction enzyme bound to DNA shows enzyme dimerization and DNA looping

Nucleic Acids Research, 2009
Atomic force microscopy (AFM) allows the study of single protein–DNA interactions such as those observed with the Type I Restriction–Modification systems.
Kelly J. Neaves, L. P. Cooper, John H. White, Stewart M. Carnally, D. Dryden, J. Edwardson, R. Henderson   +6 more
semanticscholar   +1 more source

Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP

Journal of Biological Researches, 2012
This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro, Sri Rahayu, T Susilawati, Soemarno Soemarno   +3 more
doaj   +1 more source

APOBEC3-mediated restriction of RNA virus replication

Scientific Reports, 2018
APOBEC3 family members are cytidine deaminases with roles in intrinsic responses to infection by retroviruses and retrotransposons, and in the control of other DNA viruses, such as herpesviruses, parvoviruses and hepatitis B virus.
A. Milewska, Eveline Kindler, Philip Vkovski, S. Żegleń, M. Ochman, V. Thiel, Z. Rajfur, K. Pyrć   +7 more
semanticscholar   +1 more source

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

FEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

Endonuclease A in Streptococcus pneumoniae: escaping from neutrophil extracellular traps (NETs) and relationship in immunogenicity

Exploration of Medicine
Streptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on ...
Marina Yusoff, Chew Chieng Yeo, Muhammad Hassan Nasir, Malik Amonov   +3 more
doaj   +1 more source

Genetic Polymorphism at Acaca Locus and Its Relationship With Productive Performances in Ettawa Crossbred Goat

Research Journal of Life Science, 2015
Research with aim to estimate genetic polymorphism at ACACA (Acetyl-coenzyme A carboxylase ) locus in Ettawa Crossbred goat wan its relationship with production traits was done at goat population in Batu, Lawang and Ampel Gading.
Sucik Maylinda, Tri Eko Susilorini, Puguh Surjowardojo   +2 more
doaj   +1 more source

Using shotgun sequence data to find active restriction enzyme genes

Nucleic Acids Research, 2008
Whole genome shotgun sequence analysis has become the standard method for beginning to determine a genome sequence. The preparation of the shotgun sequence clones is, in fact, a biological experiment.
Yu Zheng, J. Posfai, R. Morgan, Tamas Vincze, R. Roberts   +4 more
semanticscholar   +1 more source