Results 51 to 60 of about 2,927,400 (332)

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries

BioTechniques, 2016
Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang, Wayne A. Johnston, Mikael Boden, Elizabeth M.J. Gillam   +3 more
doaj   +1 more source

Methoden zur Untersuchung der Allelfrequenzen und-verteilung im Acetolactat-Synthase (ALS)-Gen bei Target-Site-Resistenzen in Echinochloa crus-galli

Julius-Kühn-Archiv, 2020
Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian, Keil, Sven, Löbmann, Anja, Petersen, Jan, Brändle, Frank   +4 more
doaj   +1 more source

Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)

PeerJ, 2019
Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing
N. Bayona‐Vásquez, T. Glenn, Troy J. Kieran, Todd W. Pierson, Sandra L. Hoffberg, P. A. Scott, K. Bentley, J. W. Finger, Swarnali Louha, Nicholas J. Troendle, P. Díaz‐Jaimes, R. Mauricio, B. Faircloth   +12 more
semanticscholar   +1 more source

Nucleoside triphosphate-dependent restriction enzymes [PDF]

Nucleic Acids Research, 2001
The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
Dryden, DTF, Murray, Noreen E, Rao, DN
openaire   +4 more sources

Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme Genotyping-by-Sequencing Approach

PLoS ONE, 2012
Advancements in next-generation sequencing technology have enabled whole genome re-sequencing in many species providing unprecedented discovery and characterization of molecular polymorphisms. There are limitations, however, to next-generation sequencing
J. Poland, P. Brown, M. Sorrells, J. Jannink   +3 more
semanticscholar   +1 more source

Atomic force microscopy of the EcoKI Type I DNA restriction enzyme bound to DNA shows enzyme dimerization and DNA looping

Nucleic Acids Research, 2009
Atomic force microscopy (AFM) allows the study of single protein–DNA interactions such as those observed with the Type I Restriction–Modification systems.
Kelly J. Neaves, L. P. Cooper, John H. White, Stewart M. Carnally, D. Dryden, J. Edwardson, R. Henderson   +6 more
semanticscholar   +1 more source

APOBEC3-mediated restriction of RNA virus replication

Scientific Reports, 2018
APOBEC3 family members are cytidine deaminases with roles in intrinsic responses to infection by retroviruses and retrotransposons, and in the control of other DNA viruses, such as herpesviruses, parvoviruses and hepatitis B virus.
A. Milewska, Eveline Kindler, Philip Vkovski, S. Żegleń, M. Ochman, V. Thiel, Z. Rajfur, K. Pyrć   +7 more
semanticscholar   +1 more source

Restriction & Modification Enzymes

, 2022
Restriction and modification enzymes serve as nature’s genetic gatekeepers. These specialized proteins function together as a defence mechanism in bacteria and protect them from invading foreign DNA, such as that of bacteriophages. Restriction enzymes, also known as restriction endonucleases, recognize specific sequences within the DNA and cleave it at
openaire   +2 more sources

Restriction enzyme digestion of hemimethylated DNA [PDF]

Nucleic Acids Research, 1981
Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar, Aharon Razin, Yosef Gruenbaum   +2 more
openaire   +3 more sources

Using shotgun sequence data to find active restriction enzyme genes

Nucleic Acids Research, 2008
Whole genome shotgun sequence analysis has become the standard method for beginning to determine a genome sequence. The preparation of the shotgun sequence clones is, in fact, a biological experiment.
Yu Zheng, J. Posfai, R. Morgan, Tamas Vincze, R. Roberts   +4 more
semanticscholar   +1 more source