Results 51 to 60 of about 3,197,803 (398)

Type I restriction enzymes and their relatives [PDF]

Nucleic Acids Research, 2013
Type I restriction enzymes (REases) are large pentameric proteins with separate restriction (R), methylation (M) and DNA sequence-recognition (S) subunits. They were the first REases to be discovered and purified, but unlike the enormously useful Type II REases, they have yet to find a place in the enzymatic toolbox of molecular biologists.
Loenen, Wil A M, Dryden, David T F, Raleigh, Elisabeth A, Wilson, Geoffrey G   +3 more
openaire   +4 more sources

Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao

Brazilian Archives of Biology and Technology, 2008
The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the
Francis Julio Fagundes Lopes, Marisa Vieira de Queiroz, Juliana Oliveira Lima, Viviane Aline Oliveira Silva, Elza Fernandes de Araújo   +4 more
doaj   +1 more source

MOLECULAR IDENTIFICATION AND CLONAL RELATION OF ATYPICAL ISOLATE Aeromonas salmonicida USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) [PDF]

, 2011
Aeromonas salmonicida is responsible in many cases of furunculosis outbreaks resulting in economic loss of freshwater aquaculture. Four isolates of A.
Indaryulianto, Indaryulianto, Kurniasih, Kurniasih, Priyatna, Riza   +2 more
core   +4 more sources

Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme Genotyping-by-Sequencing Approach

PLoS ONE, 2012
Advancements in next-generation sequencing technology have enabled whole genome re-sequencing in many species providing unprecedented discovery and characterization of molecular polymorphisms. There are limitations, however, to next-generation sequencing
J. Poland, P. Brown, M. Sorrells, J. Jannink   +3 more
semanticscholar   +1 more source

REBASE--restriction enzymes and DNA methyltransferases [PDF]

Nucleic Acids Research, 2004
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Roberts, Richard J., Vincze, Tamas, Posfai, Janos, Macelis, Dana   +3 more
openaire   +2 more sources

"A PCR-RFLP Method to Identification of the Important Opportunistic Fungi: Candida Species, Cryptococcus neoformans, Aspergillus famigatus and Fusarium solani" [PDF]

Iranian Journal of Public Health, 2001
Deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. DNA-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy.
SH Mirhendi, P Kordbacheh, B Kazemi, S Samiei, M Pezeshki, MR Khorramizadeh   +5 more
doaj   +1 more source

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries

BioTechniques, 2016
Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang, Wayne A. Johnston, Mikael Boden, Elizabeth M.J. Gillam   +3 more
doaj   +1 more source

Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector

Viruses, 2022
Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field.
Xiaojuan Guo, Yangyang Sun, Juan Chen, Xiaohui Zou, Wenzhe Hou, Wenjie Tan, Tao Hung, Zhuozhuang Lu   +7 more
doaj   +1 more source

Controlling the enzymatic activity of a restriction enzyme by light

Proceedings of the National Academy of Sciences of the United States of America, 2009
For many applications it would be desirable to be able to control the activity of proteins by using an external signal. In the present study, we have explored the possibility of modulating the activity of a restriction enzyme with light. By cross-linking
B. Schierling, A. Noël, W. Wende, L. T. Hien, E. Volkov, E. Kubareva, T. Oretskaya, M. Kokkinidis, A. Römpp, B. Spengler, A. Pingoud   +10 more
semanticscholar   +1 more source

Nucleoside triphosphate-dependent restriction enzymes [PDF]

Nucleic Acids Research, 2001
The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
Dryden, DTF, Murray, Noreen E, Rao, DN
openaire   +4 more sources