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Commonality of Mechanism in Glycoside Hydrolases, Nucleoside Hydrolases, and Phosphorylases: Importance of Side-Chain Conformation Preorganization. [PDF]
JACS AuTseng PS, Quirke JCK, Lin WJ, Crich D.
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Restriction & Modification Enzymes
2022Restriction and modification enzymes serve as nature’s genetic gatekeepers. These specialized proteins function together as a defence mechanism in bacteria and protect them from invading foreign DNA, such as that of bacteriophages. Restriction enzymes, also known as restriction endonucleases, recognize specific sequences within the DNA and cleave it at
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ATP-dependent restriction enzymes
2000The phenomenon of restriction and modification (R-M) was first observed in the course of studies on bacteriophages in the early 1950s. It was only in the 1960s that work of Arber and colleagues provided a molecular explanation for the host specificity.
Rao, Desirazu N +2 more
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Engineered Calcium-Precipitable Restriction Enzyme
ACS Synthetic Biology, 2014We have developed a simple system for tagging and purifying proteins. Recent experiments have demonstrated that RTX (Repeat in Toxin) motifs from the adenylate cyclase toxin gene (CyaA) of B. pertussis undergo a conformational change upon binding calcium, resulting in precipitation of fused proteins and making this method a viable alternative for ...
Josephina, Hendrix +9 more
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Restriction enzymes are bacterial enzymes that cleave DNA at specific recognition sequences, usually consisting of four to eight base pairs. These enzymes have become invaluable tools in molecular biology, enabling scientists to manipulate and analyze DNA in various ways. Restriction enzymes are used in various applications, including gene cloning, DNA
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Restriction Enzyme Computation
2003In this paper implementation of an inner hair cell model, including macromechanics and mechanical to neural transduction process is presented and discussed. The well-known Meddis model will be use as the reference system, and a high level synthesis will provide a parametrizable implementation and a reusability code, which allows future refinements on ...
Olgierd Unold, Maciej Troć
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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
Gene, 1989R. Horton +4 more
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Model for wandering restriction enzymes
Nature, 1974I WISH to propose a model for those DNA restriction enzymes which, although requiring specific recognition sites, make a limited number of cuts at apparently random sites, far from the recognition sites.
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