Results 301 to 310 of about 3,087,992 (329)
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Model for wandering restriction enzymes
Nature, 1974I WISH to propose a model for those DNA restriction enzymes which, although requiring specific recognition sites, make a limited number of cuts at apparently random sites, far from the recognition sites.
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[21] Restriction enzymes: Properties and use
1992Publisher Summary This chapter lists all commercially available enzymes, to describe their general properties, the basic procedures for their use, and ways of troubleshooting problems encountered in their use. Although the number of enzymes being discussed is large, a few simple rules apply to virtually all the enzymes.
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Understanding Restriction Enzyme Digests
2021Prior to submitting genomic DNA for sequencing, SEA students perform a restriction endonuclease digest with several enzymes, which provides a fast and cost-effective way of quickly screening isolated phages. The pattern of bands on the gel should be unique except for very closely related or identical phages, which is useful when trying to identify ...
Dustin Edwards +4 more
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Caloric restriction, aging, and antioxidant enzymes
Mutation Research/DNAging, 1993The basic mechanisms of aging and its retardation by caloric restriction (CR) remain unclear. One suggested means by which CR could retard aging is based on production of mitochondrial free radicals, and efficiency of their subsequent metabolism. Currently, there is little information concerning the influences of age and CR on the rates of in vivo ...
Ronald W. Hart +2 more
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Refining Restriction Enzyme Genome Maps
Constraints, 1997zbMATH Open Web Interface contents unavailable due to conflicting licenses.
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Restriction Enzyme Analysis of PCR Products
2009Progress in single nucleotide polymorphism (SNP) detection technologies has provided information for SNP-based studies, such as identification of candidate genes for the complex genetic diseases, pharmacogenetic analysis, drug development, population genetics, evolutionary studies, and forensic investigations. SNP detection is performed by many methods,
Hideki Asamura +3 more
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Restriction Enzymes from Thermophiles
2013Restriction endonucleases (REases) are enzymes that recognize and cleave DNA in a sequence specific manner. The recognition site consists of a sequence of nucleotides in the DNA duplex, typically four to eight base pairs long. Most of the commercially produced REases are isolated from the mesophilic bacteria.
Prince Sharma +2 more
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High Throughput Cloning with Restriction Enzymes
2008The systematic structural analysis of many target proteins involves generating expression clones in high throughput. This requires robust laboratory procedures and benefits from laboratory automation and data management systems. This chapter gives an overview of the Protein Structure Factory, a structural genomics project focusing on human proteins ...
Asgar Ergin +2 more
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Protein Engineering of Restriction Enzymes
2004Restriction endonucleases are among the most accurate enzymes known (Pingoud and Jeltsch 2001). Consequently, changing their very high sequence specificity is one of the scientific goals in studying these enzymes (Jeltsch et al. 1996). This review focuses on protein engineering regarding Type II restriction enzymes.
Jürgen Alves, P. Vennekohl
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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
Gene, 1989R. Horton +4 more
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