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Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism [PDF]
Diagnostics, 2019 Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes ...
María del Mar García-Suárez +5 more
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Restriction fragment length polymorphism (RFLP) [PDF]
American Journal of Physical Anthropology, 1989 A restriction fragment length polymorphism (RFLP) is defined by an enzyme, a restriction endonuclease, that cuts the doublestranded DNA at a particular sequence of bases, a probe, a labeled, complementary segment of DNA that will anneal to a portion of the digested sample, and a set of variable fragment length bands that appear on a Southern blot.
Robert C. Williams
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IDENTIFICATION OF ROSE CULTIVARS BY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS [PDF]
HortScience, 1991 Restriction Fragment Length Polymorphisms (RFLPs) were investigated in rose cultivars as a means of reliable cultivar identification. A random genomic DNA library was generated by shotgun cloning HindIII digested fragments of DNA extracted from rose cultivar Confection into pUC8 plasmid of Escherichia coli strain JM 83.
John W. Kelly +4 more
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Restriction fragment length polymorphism ofAzospirillumstrains [PDF]
FEMS Microbiology Letters, 1991 Total DNA of various Azospirillum strains representing different species was digested with restriction enzymes, Southern blotted and hybridized with four A. brasilense probes. Pairwise comparison of the conserved hybridization fragments was applied to calculate sequence divergence and to group the strains using the unweighted pair group method.
FANI, RENATO +5 more
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334 RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN CARROT [PDF]
HortScience, 1994 Studies of genetic variation at the DNA level in the genus Daucus have been very limited. Molecular markers based on restriction fragment length polymorphism (RPLP) have been shown to be highly useful and efficient gene markers in other plant species. We have used a total of 20 carrot types (inbreds, varieties, species) for this study.
Philipp W. Simon, Vivek Sampath
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Genetic Analysis of Restriction Fragment Length Polymorphisms in Vitis [PDF]
Journal of Heredity, 1992 The parents and progeny from two crosses (Cayuga White x Aurore and NY62.136.2 x Yates) were examined for the presence of DNA restriction fragment length polymorphisms (RFLPs). Seventeen independent DNA sequences were used in the analysis, 15 obtained from a grape Pstl genomic library and two heterologous probes obtained from other laboratories.
Mauro, M-C +3 more
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Restriction Fragment Length Polymorphisms in Poultry Breeding
Poultry Science, 1986 Abstract Restriction fragment length polymorphisms (RFLP) are a new class of genetic marker uncovered by means of recombinant deoxyribonucleic acid (DNA) methodologies. The RELP are formed as a result of point mutations or changes in chromosome structure that affect the distribution of restriction enzyme sites in the vicinity of a cloned gene used as
Jacques S. Beckmann, M. Soller
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Terminal Restriction Fragment Length Polymorphism Data Analysis [PDF]
Applied and Environmental Microbiology, 2003 Blackwood et al. (1) discuss the statistical analysis of terminal restriction fragment length polymorphism (T-RFLP) data. They sampled several soils and identified methods that correctly grouped replicate samples (cluster analysis) and successfully distinguished between site differences (redundancy analysis).
Grant, Alastair +5 more
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Inheritance of restriction fragment length polymorphisms in Agaricus brunnescens. [PDF]
Genetics, 1989 Abstract The cultivated mushroom, Agaricus brunnescens, is secondarily homothallic; most basidia produce only two basidiospores, each of which receives two of the four post meiotic nuclei. The segregation of restriction fragment length polymorphisms (RFLPs) detected by four plasmid probes carrying single-copy nuclear DNA of Agaricus was ...
James B. Anderson +3 more
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Mixed chimerism and restriction fragment length polymorphism [letter] [PDF]
Blood, 1987 HJ Kolb, J Mittermuller
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