Results 41 to 50 of about 2,795,666 (307)

Molecular detection and PCR-RFLP analysis using Pst1 and Alu1 of multidrug resistant Klebsiella pneumoniae causing urinary tract infection in women in the eastern part of Bangladesh

open access: yesJournal of Genetic Engineering and Biotechnology, 2018
Klebsiella pneumoniae is the second leading causative agent of UTI. In this study, a rapid combined polymerase chain reaction and restriction fragment length polymorphism analysis was developed to identify K.
Golam Mahmudunnabi   +5 more
doaj   +1 more source

Association of TLR3 single nucleotide polymorphisms with susceptibility to HTLV-1 infection in Iranian asymptomatic blood donors

open access: yesRevista da Sociedade Brasileira de Medicina Tropical, 2020
INTRODUCTION: The human T-lymphotropic virus type 1 (HTLV-1) has a single-stranded RNA genome and expresses specific proteins that have oncogenic potential. Approximately 15 to 20 million people worldwide have been infected by this virus.
Hossein Mehrabi Habibabadi   +4 more
doaj   +1 more source

Homoplasy corrected estimation of genetic similarity from AFLP bands, and the effect of the number of bands on the precision of estimation [PDF]

open access: yes, 2009
AFLP is a DNA fingerprinting technique, resulting in binary band presence–absence patterns, called profiles, with known or unknown band positions. We model AFLP as a sampling procedure of fragments, with lengths sampled from a distribution.
van Eeuwijk, F.A.   +7 more
core   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

open access: yesAdvanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad   +12 more
wiley   +1 more source

Molecular Detection and Characterisation of Grapevine Phytoplasmas in Macedonia

open access: yesJournal of Agriculture and Plant Sciences, 2013
In the period from 2006 till 2008, continually were checking the fi eld grapevine symptoms, with primary aim to confi rm presence and representation of phytoplasmas diseases in per sent. For collection of material for analyzes, we check seven region with
Sasa Mitrev   +2 more
doaj  

Polimorfismo no gene GH1-PstI associado a características corporais de linhagens de tilápia-do-nilo Polymorphism in the GH1-PstI gene associated to corporal characteristics in Nile tilapia strains

open access: yesPesquisa Agropecuária Brasileira, 2009
O objetivo deste trabalho foi determinar a associação de polimorfismos no gene do hormônio crescimento GH1 às características corporais, em linhagens de tilápia-do-nilo (Oreochromis niloticus). Foram coletados fragmentos da nadadeira caudal de exemplares
Danielly Veloso Blanck   +3 more
doaj   +1 more source

Restriction fragment length polymorphism of Azospirillum strains

open access: yes, 1991
Total DNA of various Azospirillum strains representing different species was digested with restriction enzymes, Southern blotted and hybridized with four A. brasilense probes.
Fani R   +6 more
core   +1 more source

Special features of RAD Sequencing data:implications for genotyping [PDF]

open access: yes, 2012
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Fuentes-Utrilla, Pablo   +12 more
core   +1 more source

Auto‐Downregulation of the Florigen FT Production Prevents Precocious Flowering in Plants

open access: yesAdvanced Science, EarlyView.
This study shows that the leaf vein‐expressed FD complexes with the florigen FT to bind DNA motifs in FT promoter, which antagonizes CONSTANS‐mediated FT activation to feedback down‐regulate FT expression. This prevents an excessive induction of FT expression by inductive photoperiods, thereby precluding precocious transition to flowering in ...
Shu Tian, Xiao Luo, Bowen Cui, Yuehui He
wiley   +1 more source

Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods

open access: yesJurnal Sain Peternakan Indonesia, 2022
This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method.
Teguh Rafian   +3 more
doaj   +1 more source

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