Results 51 to 60 of about 145,242 (313)

Causal Prediction of TP53 Variant Pathogenicity Using a Perturbation‐Informed Protein Language Model

Advanced Science, EarlyView.
A TP53‐specific predictor, CaVepP53, is developed by fine‐tuning ESMC on experimentally validated variants, quantifying pathogenicity via Euclidean distances. It outperforms general‐purpose models and extends to five cancer genes, enabling interpretable variant classification for precision medicine.
Huiying Chen, Yang Zhao, Boqiang Hu, Wuke Wang, Minfang Song, Annabeth Xinyu Zhao, Xiangyang Li, Gefei Wang, Yanfen Wang, Weiyan Zheng, Xinpeng Zhang, Xia Lin, Yanbin Yin, Xingxu Huang, Jinfang Zheng, Tingbo Liang   +15 more
wiley   +1 more source

Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community

Plant, Soil and Environment, 2009
A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group
J. Kopecký, G. Novotná, M. Ságová-Marečková   +2 more
doaj   +1 more source

A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California

Ciencias Marinas, 2020
The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to identify 3 species in the genus Opisthonema (Opisthonema bulleri, Opisthonema medirastre, and Opisthonema libertate) in the southern Gulf of ...
Carlos Iván Pérez-Quiñonez, Casimiro Quiñonez-Velázquez, Francisco Javier García-Rodríguez   +2 more
doaj   +1 more source

Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis [PDF]

, 2006
The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids ...
Bruce, K. D., Carroll, M. P., Connett, G. J., Hockey, P. M., Jones, G., Kehagia, V., Rogers, G. B., Serisier, D. J.   +7 more
core   +2 more sources

Cryo‐EM Structure Guided Engineering of Botulinum Neurotoxin A With Advanced Receptor Binding Affinity and Therapeutical Benefits

Advanced Science, EarlyView.
The butterfly unfolded wing in an open form structure of botulinum neurotoxin type A (BoNT/A) at physiological‐state was confirmed at 2.85 Å resolution by cryo‐electron microscopy (cryo‐EM). Structure‐guided protein engineering significantly enhanced the receptor‐binding affinity, therapeutic efficacy, and safety of the engineered toxin variants ...
Wenrui Wang, Zhaxi Zerang, Linjin You, Ziye Liu, Rong Nie, Fuwei Qi, Fenfen Gao, Chengmu Zhao, Wantong Ma, Jinghan He, Xiaoru Wang, Shanquan Wu, Bo Liu, Xinyao Liu, Dongsheng Lei, Dejuan Zhi, Dongsheng Wang   +16 more
wiley   +1 more source

Study of Calpastatin Gene Polymorphism in Holstein Cattle and Buffalo

Scientific Papers Animal Science and Biotechnologies, 2023
In this research for studying genetic polymorphism of calpastatin (CAST) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were collected from 50 Holstein cattle and 40 buffalo randomly.
Soheil Yousefi, Ahani Azari Mojtaba
doaj  

IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in Campinas, Brazil: evidence of intercontinental distribution of strains

Memorias do Instituto Oswaldo Cruz, 2003
Tuberculosis (TB) is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present in local prevalent strains of Mycobacterium tuberculosis, the distribution and clustering of
Ana Lucia Roscani Calusni, Gláucia Nogueira Roscani, Maria Cecília Barisson Villares, Hanna Soini, Edward A Graviss, Marcelo de Carvalho Ramos   +5 more
doaj   +1 more source

Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin [PDF]

, 2007
Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat ...
Chai, Lay Ching, Cheah, Yoke Kqueen, Gunsalam, Jurin Wolmon, Mat Issa @ Zakaria, Zuraini, Mohamad Ghazali, Farinazleen, Ong, Sang Bing, Radu, Son, Robin, Tunung, Yuli, Haryani   +8 more
core  

Combination Immunotherapy as a Promising Strategy to Overcome Immunotherapy Resistance: From Emergence to Next‐Generation Approaches

Advanced Science, EarlyView.
This review examines emerging combination immunotherapy strategies tailored to distinct tumor microenvironments and highlights next‐generation biomarkers that guide response prediction and treatment personalization. It integrates lessons from unsuccessful trials, addresses toxicity challenges, and outlines approaches for early biomarker discovery and ...
Asmita Pandey, Sudhir Kumar Rai, Li Ma, Lauren Higa, Vedbar Khadka, Hua Yang, Youping Deng   +6 more
wiley   +1 more source

Diagnosis of Aspergillus fumigatus endophthalmitis from formalin fixed paraffin-embedded tissue by polymerase chain reaction-based restriction fragment length polymorphism

Indian Journal of Ophthalmology, 2008
New molecular biological technique of Polymerase Chain Reaction (PCR) based Restriction Fragment Length Polymorphism (RFLP) can identify the species from paraffin-embedded tissue section.
Biwas Jyotirmay, Bagyalakshmi R, Therese Lily   +2 more
doaj