Results 61 to 70 of about 2,795,666 (307)

IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in Campinas, Brazil: evidence of intercontinental distribution of strains

open access: yesMemorias do Instituto Oswaldo Cruz, 2003
Tuberculosis (TB) is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present in local prevalent strains of Mycobacterium tuberculosis, the distribution and clustering of
Ana Lucia Roscani Calusni   +5 more
doaj   +1 more source

A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California

open access: yesCiencias Marinas, 2020
The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to identify 3 species in the genus Opisthonema (Opisthonema bulleri, Opisthonema medirastre, and Opisthonema libertate) in the southern Gulf of ...
Carlos Iván Pérez-Quiñonez   +2 more
doaj   +1 more source

Restriction fragment length polymorphism (RFLP) [PDF]

open access: yesAmerican Journal of Physical Anthropology, 1989
A restriction fragment length polymorphism (RFLP) is defined by an enzyme, a restriction endonuclease, that cuts the doublestranded DNA at a particular sequence of bases, a probe, a labeled, complementary segment of DNA that will anneal to a portion of the digested sample, and a set of variable fragment length bands that appear on a Southern blot.
openaire   +1 more source

Use of restriction enzyme fragment length polymorphism (RFLP) of the 16S-23S rRNA internal transcribed spacer region (ITS) for identification of fish mycobacteria

open access: yes, 2013
PCR targeting the 16S-23S rRNA gene internally transcribed spacer (ITS) region has been proposed as a rapid and reliable method for the detection of Mycobacterium species in human clinical specimens.
Pourahmad, Fazel, Richards, Randolph
core   +1 more source

Integrative Omics Analysis Reveals the Regulation of Hypoxia Tolerance in Large Yellow Croaker (Larimichthys crocea) via the Lipoic Acid Synthase (lias) Gene

open access: yesAdvanced Science, EarlyView.
Lipoic acid synthase (lias) can regulate α‐KG levels through lipoylation, thereby negatively regulating HIF‐1α protein levels via PHD under hypoixa. The Hap2 allele of lias exhibits lower expression levels than Hap1, leading to the accumulation of more HIF‐1α protein and thereby enhancing hypoxia tolerance. ABSTRACT Hypoxia stress seriously affects the
Jie Ding   +7 more
wiley   +1 more source

Análisis de genotipos del virus de la hepatitis B mediante el análisis de polimorfismos de longitud de fragmentos de restricción

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2016
Introducción. Se han descrito diez genotipos (A-J) del virus de la hepatitis B (HBV) que están distribuidos en todos los continentes. Una de las técnicas utilizadas para determinar el genotipo viral es el análisis del polimorfismo de longitud de los ...
Julio-C Rendón   +4 more
doaj   +1 more source

Whole‐Genome Sequencing Pilot of the Central Asian Genomic Diversity Project Reveals Distinct Histories, Adaptation, and Introgression

open access: yesAdvanced Science, EarlyView.
As a pilot phase of the Central Asian Genomic Diversity Project, whole‐genome sequencing of 166 individuals from 20 Central Asian and Afghan Hazara populations reveals fine‐scale substructure shaped by repeated trans‐Eurasian migration and admixture. Integrated analyses uncover post‐admixture adaptation, archaic introgression, and medically relevant ...
Mengge Wang   +11 more
wiley   +1 more source

EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM [PDF]

open access: yesPakistan Veterinary Journal, 2008
In the camel tyrosinase gene, a restriction site provoked by the T variant was used in a special restriction fragment length polymorphism analysis (PCR-RFLP) for genotyping of animals from six different Pakistani camel breeds (Marecha, Dhatti, Larri ...
M. G. SHAH, M. REISSMANN1, A. S. Qureshi2 AND H. J. SCHWARTZ3
doaj  

Restriction fragment length polymorphism of the Vibrio anguillarum serovar 01 virulence plasmid

open access: yes, 1990
Seventy-eight strains of Vibrio anguillarum serovar 01, all harboring one 65- to 70-kilobase plasmid, were typed according to restriction fragment length polymorphism of the plasmid.
Olsen, J. E.
core   +1 more source

Multi‐tissue Metabolic GWAS and Drought‐Responsive Multi‐omics Reveal the Genetic Basis of the Quinoa Metabolome

open access: yesAdvanced Science, EarlyView.
A multi‐omics framework combining multitissue genome‐wide association studies, metabolomics, transcriptomics, proteomics, and functional validation uncovers the genetic basis of specialized metabolism in quinoa. The study identifies hundreds of metabolite‐associated loci, prioritizes candidate genes for saponin, betalain, and flavonoid biosynthesis ...
Julia von Steimker   +11 more
wiley   +1 more source

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