This assay enables multiplex genotyping of HLA‐CREGs and HPA alleles from whole blood using qPCR with an integrated parallel negative control. Results are interpreted based on amplification curves and Ct values, with validity dependent on strict quality control criteria for both the target reactions and the negative control.
Huimin Ji +9 more
wiley +1 more source
A simple method for detection of a novel coronavirus (SARS-CoV-2) using one-step RT-PCR followed by restriction fragment length polymorphism. [PDF]
Son HA +15 more
europepmc +1 more source
Abstract BACKGROUND Rice milling characteristics and storage stability are critical traits influencing industrial applications. Floury endosperm rice carrying the flo4‐4 allele is valuable for dry milling but exhibits reduced storage stability associated with altered starch structural characteristics.
Su‐Kyung Ha +11 more
wiley +1 more source
Methodological Aspects of Multiplex Terminal Restriction Fragment Length Polymorphism-Technique to Describe the Genetic Diversity of Soil Bacteria, Archaea and Fungi. [PDF]
Gryta A, Frąc M.
europepmc +1 more source
The Swedish Arctic Fox Project: 40 years of research in ecology and conservation
The 4 central research themes in The Swedish Arctic Fox Project and key deliverables for practical conservation achieved over 4 decades. Abstract The scientific community widely recognizes the value of long‐term studies, especially those with individual‐level resolution; however, maintaining a consecutive data series is associated with multiple ...
Anders Angerbjörn +11 more
wiley +1 more source
Discrimination of Hard Ticks by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). [PDF]
Perumalsamy N +4 more
europepmc +1 more source
ABSTRACT Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation and progressive joint damage. Although ferroptosis has been implicated in RA progression, the role of disulfidptosis and its interaction with ferroptosis remains unclear.
Ting‐Ting Wang +10 more
wiley +1 more source
Chromatic Variants of Pityriasis Versicolor and Molecular Species Identification Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). [PDF]
Romero-Navarrete M +8 more
europepmc +1 more source
Frequency of Candida Species in the Oral Cavity of Narcotics and Stimulants Smokers in Isfahan, Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method. [PDF]
Abharian PH +3 more
europepmc +1 more source
Ion Activation Methods for Top‐Down Proteomics
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source

