Results 21 to 30 of about 63,405 (314)

DNA Fingerprinting of Resistant Mycobacterium tuberculosis Isolates in Iran by IS6110-RFLP Method

Galician Medical Journal, 2019
The objective of the research was to identify resistant Mycobacterium tuberculosis strains and recognize their molecular epidemiology and how they are disseminated in order to determine the cause and the effect of drug resistance and the process of its ...
Aliasghar Farazi, Masoomeh Sofian, Rohollah Keshavarz, Shima Malekmohammadi   +3 more
doaj   +1 more source

MOLECULAR CHARACTERIZATION OF ‘Candidatus PHYTOPLASMA MALI’ STRAINS FROM BULGARIA AND POLAND

Acta Scientiarum Polonorum: Hortorum Cultus, 2019
During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of
Mirosława Cieślińska, Aneliya Borisova   +1 more
doaj   +1 more source

334 RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN CARROT [PDF]

HortScience, 1994
Studies of genetic variation at the DNA level in the genus Daucus have been very limited. Molecular markers based on restriction fragment length polymorphism (RPLP) have been shown to be highly useful and efficient gene markers in other plant species. We have used a total of 20 carrot types (inbreds, varieties, species) for this study.
Philipp W. Simon, Vivek Sampath
openaire   +1 more source

Autophagy in cancer and protein conformational disorders

FEBS Letters, EarlyView.
Autophagy plays a crucial role in numerous biological processes, including protein and organelle quality control, development, immunity, and metabolism. Hence, dysregulation or mutations in autophagy‐related genes have been implicated in a wide range of human diseases.
Sergio Attanasio
wiley   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

MET and NF2 alterations confer primary and early resistance to first‐line alectinib treatment in ALK‐positive non‐small‐cell lung cancer

Molecular Oncology, EarlyView.
Alectinib resistance in ALK+ NSCLC depends on treatment sequence and EML4‐ALK variants. Variant 1 exhibited off‐target resistance after first‐line treatment, while variant 3 and later lines favored on‐target mutations. Early resistance involved off‐target alterations, like MET and NF2, while on‐target mutations emerged with prolonged therapy.
Jie Hu, Ning Ding, Xiaobo Xu, Yedan Chen, Yong Zhang, Jingwen Liu, Jiebai Zhou, Hairong Bao, Donghui Zhang, Yijun Song, Yang Shao, Yuanlin Song   +11 more
wiley   +1 more source

A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics

BMC Research Notes, 2018
Objective This study describes a restriction fragment polymorphism protocol for rapidly screening the polymorphism SLCO1B1 c.1929A>C in genomic DNA samples.
Nyarai D. Soko, Collen Masimirembwa, Collet Dandara   +2 more
doaj   +1 more source

Genetic Analysis of Restriction Fragment Length Polymorphisms in Vitis [PDF]

Journal of Heredity, 1992
The parents and progeny from two crosses (Cayuga White x Aurore and NY62.136.2 x Yates) were examined for the presence of DNA restriction fragment length polymorphisms (RFLPs). Seventeen independent DNA sequences were used in the analysis, 15 obtained from a grape Pstl genomic library and two heterologous probes obtained from other laboratories.
Mauro, M-C, Strefeler, M, Weeden, N, Reisch, B   +3 more
openaire   +2 more sources

Genomics‐led approach to drug testing in models of undifferentiated pleomorphic sarcoma

Molecular Oncology, EarlyView.
GA text Genomic data from undifferentiated pleomorphic sarcoma patients and preclinical models were used to inform a targeted drug screen. Selected compounds were tested in 2D and 3D cultures of UPS cell lines. A combination of trametinib and infigratinib was synergistic in the majority of UPS cell lines tested, which was further confirmed in an ex ...
Piotr J. Manasterski, Molly R. Danks, John P. Thomson, Morwenna Muir, Martin Lee, John C. Dawson, Ana T. Amaral, Juan Diaz‐Martin, David S. Moura, Javier Martin‐Broto, Ali Alsaadi, Donald M. Salter, Ailsa J. Oswald, Graeme Grimes, Larry Hayward, Ted R. Hupp, Karen Sisley, Paul H. Huang, Neil O. Carragher, Valerie G. Brunton   +19 more
wiley   +1 more source

IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE

Ribogospodarsʹka Nauka Ukraïni, 2013
The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency
Yu. Rud
doaj   +1 more source