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Reverse Genetics of Zika Virus
2017We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A
Chao, Shan, Xuping, Xie, Pei-Yong, Shi
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2017
The Old World (OW) arenavirus Lassa (LASV ) is estimated to infect several hundred thousand people yearly in West Africa, resulting in high numbers of Lassa fever (LF), a viral hemorrhagic fever (HF) disease associated with high morbidity and mortality.
Luis, Martínez-Sobrido +2 more
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The Old World (OW) arenavirus Lassa (LASV ) is estimated to infect several hundred thousand people yearly in West Africa, resulting in high numbers of Lassa fever (LF), a viral hemorrhagic fever (HF) disease associated with high morbidity and mortality.
Luis, Martínez-Sobrido +2 more
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Chloroplast Transformation and Reverse Genetics
2006For chloroplast transformation, the most efficient method to introduce DNA is particle bombardment. To then select the cells which harbor a transformed plastid, two classes of markers are available. With one class, selection is based on the rescue of a non-photosynthetic mutant with the wild-type chloroplast gene.
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‘REVERSE GENETICS’ IN PETUNIA HYBRIDA
2001Petunia hybrida is a suitable plant system for reverse genetics ...
TORNIELLI, Giovanni Battista +5 more
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Reverse Genetics of Influenza Virus
2014Reverse genetics is the creation of a virus from a full-length cDNA copy of the viral genome, referred to as an "infectious clone," and is one of the most powerful genetic tools in modern virology. Since its development in 1999, plasmid-based reverse genetics has been effectively applied to numerous aspects of influenza studies which include ...
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Rotavirus reverse genetics systems: Development and application
Virus Research, 2021Yuta Kanai, Takeshi Kobayashi
exaly
SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract
Cell, 2020Scott H Randell, Alain C Borczuk
exaly
FAST Proteins: Development and Use of Reverse Genetics Systems for Reoviridae Viruses
Annual Review of Virology, 2021Yuta Kanai, Takeshi Kobayashi
exaly