Results 201 to 210 of about 335,945 (253)

Reverse Genetics of TheParamyxoviridae

1999
Publisher Summary This chapter describes the experiments using the technology of nonsegmented negative-strand virus genome rescue in paramyxoviruses, which led to the recovery of infectious virus from complementary DNA (cDNA) and considers the rescue of infectious virus from the members of each of the four genera for which it has been achieved ...
A C, Marriott, A J, Easton
openaire   +2 more sources

Reverse Genetics of Filoviruses

2017
Reverse genetics systems are used for the generation of recombinant viruses. For filoviruses, this technology has been available for more than 15 years and has been used to investigate questions regarding the molecular biology, pathogenicity, and host adaptation determinants of these viruses.
Thomas, Hoenen, Janine, Brandt, Yíngyún, Caì, Jens H, Kuhn, Courtney, Finch   +4 more
openaire   +2 more sources

Reverse Genetics of Nodaviruses

1999
Publisher Summary This chapter focuses on the reverse genetics of Nodaviruses. With the construction of full-length complementary DNA (cDNA) clones of the two genomic RNAs of an alpha nodavirus, it became possible to address all stages of the virus life cycle using reverse genetic methods.
L A, Ball, K L, Johnson
openaire   +2 more sources

Forward in Reverse: How Reverse Genetics Complements Chemical Genetics

Pharmacogenomics, 2005
Pharmaceutical discovery has a renewed interest in physiological screening, while chemical genomics initiatives will soon provide a large amount of cellular assay data. However, there is no single robust approach to connect active compounds with their targets, limiting their experimental and therapeutic use.
openaire   +2 more sources

Reverse Genetics of Dengue Virus

2023
Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV biology, which have been facilitated by the development of different molecular strategies for ...
José Valter Joaquim, Silva Júnior, Andréa Nazaré Monteiro Rangel, da Silva, Jefferson José, da Silva Santos, Laura Helena Vega Gonzales, Gil   +3 more
openaire   +2 more sources

Reverse Genetics of Picornaviruses

1999
Publisher Summary This chapter focuses on the reverse genetics of picornaviruses. The family Picornaviridae includes a large number of human and animal pathogens, all related by the possession of a small, nonenveloped, icosahedral particle and a single-stranded, positive-sense, RNA genome.
openaire   +2 more sources

Reverse Genetics of Mononegavirales

2004
"Reverse genetics" or de novo synthesis of nonsegmented negative-sense RNA viruses (Mononegavirales) from cloned cDNA has become a reliable technique to study this group of medically important viruses. Since the first generation of a negative-sense RNA virus entirely from cDNA in 1994, reverse genetics systems have been established for members of most ...
openaire   +2 more sources

Genetics and Reverse Genetics of Nidoviruses

2014
The genomes of nidoviruses are infectious, and virus replication is initiated as the genome is delivered to the cytoplasm and the replicase is translated by the host cell ribosomes. Nidovirus reverse-genetics systems are needed to better understand aspects of their complex replication strategy, pathogenesis, and mechanisms of host range expansion, and ...
Damon J. Deming, Ralph S. Baric
openaire   +1 more source

Genetic programming for Reverse Engineering

2013 20th Working Conference on Reverse Engineering (WCRE), 2013
This paper overviews the application of Search Based Software Engineering (SBSE) to reverse engineering with a particular emphasis on the growing importance of recent developments in genetic programming and genetic improvement for reverse engineering.
Mark Harman, William B. Langdon, Westley Weimer   +2 more
openaire   +1 more source

Reverse Genetics Systems for Filoviruses

2023
Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable.
Bianca S, Bodmer, Thomas, Hoenen
openaire   +2 more sources