Controls for reverse transcriptase-polymerase chain reaction amplification of BCR-ABL transcripts [letter] [PDF]
JV Melo, NS Kent, XH Yan, JM Goldman
openalex +1 more source
What's New? Integrins are critical mediators of cell adhesion and migration and have been implicated in cancer progression. Here, the authors show that endothelial cells in target organs of colorectal cancer metastasis express fibronectin, and that colorectal cancer tumour cells can bind fibronectin through integrin β6. This interaction is resistant to
Chiara Van Passen+16 more
wiley +1 more source
Parechovirus and enteroviruses among young infants with sepsis in Iran
Background and Objectives: Human parechoviruses (HPeV) and Human enteroviruses (EV) frequently cause a sepsis-like illness in young infants (younger than three months).
Manoochehr Makvandi+6 more
doaj
Case series - COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis. [PDF]
Masterson JM+9 more
europepmc +1 more source
Simultaneous Qualitative And Quantitative Identification Of Multiple Targets By Polymerase Chain Reaction On Microarray [PDF]
An approach for multiplex qualitative and quantitative microarray-based PCR analysis has been proposed. The characteristics of PCR executed on a gel-based oligonucleotide microarray with immobilized forward primers and a single common reverse primer in solution were investigated for several DNA targets.
arxiv
Failure to detect BCR-ABL message in some Ph+ hematopoietic colonies: methodological aspects of the reverse transcriptase-polymerase chain reaction assay [letter; comment] [PDF]
T Maekawa+4 more
openalex +1 more source
Competitive Reverse-Transcriptase Polymerase Chain Reaction without an Artificial Internal Standard
Advances in our understanding of molecular and cellular physiology necessitate that mRNA levels for specific growth factors and other rare transcripts be measured quantitatively in small samples. Conventional methods such as Northern blot analysis and solution hybridization/ribonuclease protection are not sufficiently sensitive.
Alan R. Shuldiner+4 more
openaire +3 more sources
This research presents a method based on nanopore targeted sequencing (NTS) with pathogen‐specific panels for testing infective endocarditis (IE) and myocarditis. The NTS achieved a detection limit of 20 copies/test for the IE target panel and 10 copies/test for the myocarditis target panel.
Aisi Fu+10 more
wiley +1 more source
Until recently, two clades of of avian influenza viruses (AIVs) designated as 2.3.2 and 2.2.3 havebeen circulating in Indonesia. Mutations of AIV genes have cretaed many more variants of the virus.
Ni Luh Putu Indi Dharmayanti+2 more
doaj
A Chemical Kinetic Model of Transcriptional Elongation [PDF]
A chemical kinetic model of the elongation dynamics of RNA polymerase along a DNA sequence is introduced. The proposed model governs the discrete movement of the RNA polymerase along a DNA template, with no consideration given to elastic effects. The model's novel concept is a ``look-ahead'' feature, in which nucleotides bind reversibly to the DNA ...
arxiv