Comparison of Northern blot hybridization and a reverse transcriptase-polymerase chain reaction technique for measurement of mRNA expression of metalloproteinases and matrix components in articular cartilage and synovial membrane from horses with osteoarthritis [PDF]
Joanne E. Fehr+3 more
openalex +1 more source
MUSTN1 Interaction With SMPX Regulates Muscle Development and Regeneration
Model of the role of MUSTN1 in skeletal muscle development. MUSTN1‐KO mice display delayed muscle growth and regeneration. MUSTN1 protects SMPX from instability and maintains the morphology of muscle fibres. MUSTN1 and SMPX synergistically promote myoblast proliferation and dependently facilitate differentiation.
Yu Fu+6 more
wiley +1 more source
Short communication: optimization of semi-quantitative RT PCR analysis for CPT I gene expression in rainbow trout (Oncorhynchus mykiss) [PDF]
A key enzyme in mitochondrial β¬oxidation, carnitine palmitoyltransferase (CPT) I, is transcriptionally regulated in mammals, but this enzyme also experiences allosteric modulations (Harano et al., 1985; Murthy and Pande, 1987; Bezaire et al., 2004). CPT
Jalali, S.A.H.+3 more
core
Heparin interference with reverse transcriptase polymerase chain reaction of RNA extracted from lungs after ischemia-reperfusion [PDF]
Xiaohui Bai+3 more
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Illustration of the preparation of tFNAs‐DJ‐1 saRNAs complex and its inhibitory effects on ferroptosis in R28 and retina. ABSTRACT Retinal ischaemia/reperfusion injury (RI/RI) is the primary pathophysiological mechanism underlying retinal ischaemic diseases, potentially resulting in significant and irreversible visual impairment.
Xianggui Zhang+10 more
wiley +1 more source
Genetic analysis of the 2019 coronavirus pandemic with from real-time reverse transcriptase polymerase chain reaction. [PDF]
Farasani A.
europepmc +1 more source
A new approach to understanding T cell development: the isolation and characterization of immature CD4-, CD8-, CD3- T cell cDNAs by subtraction cloning [PDF]
During T cell development in the mammalian thymus, immature T cells are observed that lack the cell surface markers CD4, CD8, and CD3. A subtracted cDNA library was constructed to isolate cDNAs that are specific for these immature T cells.
Gese, Elaine, Hood, Leroy, Orr, Sally L.
core
Differential Role for Competitive Reverse Transcriptase-Polymerase Chain Reaction and Intracellular Cytokine Staining as Diagnostic Tools for the Assessment of Intragraft Cytokine Profiles in Rejecting and Nonrejecting Heart Allografts [PDF]
Bernd M. Spriewald+5 more
openalex +1 more source