Results 211 to 220 of about 172,121 (249)

Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction

Molecular and Biochemical Parasitology, 2000
A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript ...
M, Menegon, C, Severini, A, Sannella, M G, Paglia, D, Sangaré, A, Abdel-Wahab, A A, Abdel-Muhsin, H, Babiker, D, Walliker, P, Alano   +9 more
openaire   +2 more sources

Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Hongxin, Fan, Ryan S, Robetorye
openaire   +2 more sources

Detection of measles virus by reverse-transcriptase polymerase chain reaction in a placenta

The Journal of Maternal-Fetal & Neonatal Medicine, 2009
Measles virus (MV) during pregnancy is associated with maternal morbidity and mortality and can put the fetus and newborn at risk of a wide range of complications. Reverse-transcriptase polymerase chain reaction (RT-PCR) for detecting MV in the placenta has not been reported.A case of RT-PCR detection of MV in the placenta of a 38-year-old woman who ...
Shikma, Bar-On, Yifat, Ochshorn, Ora, Halutz, Yair, Aboudy, Ariel, Many   +4 more
openaire   +2 more sources

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

2003
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene ...
openaire   +2 more sources

Detection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction

2003
In oncology, the correct assessment of tumor stage is crucial information, as it relates to disease-free interval and prognosis. Routine staging modalities use histopathology to evaluate the presence or absence of metastasis mainly in tissues such as lymph nodes, as described in Chapter 1 by Roskell and Buley.
K, Haack, K, Soondrum, M, Neumaier
openaire   +2 more sources

Early diagnosis of murray valley encephalitis by reverse transcriptase-polymerase chain reaction

Pathology, 2000
A 4-year-old aboriginal boy developed encephalitis due to Murray Valley encephalitis virus (MVE) following an earlier infection with Kunjin virus (KUN). The illness was severe, resulting in cerebral atrophy and profound physical and intellectual disability.
P C, McMinn, P G, Carman, D W, Smith
openaire   +2 more sources

Detection and Identification of Flaviviruses by Reverse Transcriptase Polymerase Chain Reaction

1992
We developed a reverse transcriptase/polymerase chain reaction (RT/PCR) for the detection and identification of flavivirus RNA in a single-tube assay. Universal flavivirus RT/PCR amplimers amplify an 832-bp region in the carboxyl portion of NS5 (nt9166-9997) which contains nucleotide sequences conserved among all flaviviruses at the 3′- and 5 ...
Dennis W. Trent, Gwong-Jen Chang
openaire   +1 more source

A reverse transcriptase‐polymerase chain reaction assay in the diagnosis of soft tissue sarcomas

Cancer, 2000
Many types of sarcomas are characterized by specific chromosomal translocations that result in the production of novel chimeric genes. Detection of these fusion genes could be a sensitive molecular diagnostic assay. However, to the authors' knowledge there have been few systemic comparisons between the current histopathologic diagnosis and the presence
N, Naito, A, Kawai, M, Ouchida, T, Dan'ura, Y, Morimoto, T, Ozaki, K, Shimizu, H, Inoue   +7 more
openaire   +2 more sources

Quantification of poliovirus in seawater and sewage by competitive reverse transcriptase - polymerase chain reaction

Canadian Journal of Microbiology, 1998
Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time. However, unlike traditional cell culture, RT-PCR has not provided quantitation and infectivity information.
Y L, Tsai, S L, Parker
openaire   +2 more sources

Detection of Rotaviruses in the Day Care Environment by Reverse Transcriptase Polymerase Chain Reaction

Journal of Infectious Diseases, 1992
Group A rotavirus is an important cause of morbidity among infants and toddlers in day care centers. Transmission by the fecal-oral route is well established, but fomites and environmental surfaces may also play an important role in transmission. A highly sensitive polymerase chain reaction (PCR) assay was used to detect rotavirus RNA in day care ...
J, Wilde, R, Van, L, Pickering, J, Eiden, R, Yolken   +4 more
openaire   +2 more sources