Results 201 to 210 of about 172,121 (249)

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

Angewandte Chemie, Volume 138, Issue 29, 13 July 2026.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter, Harmen J. van der Veer, Sterre de Lignie, Robbert J. de Haas, Renko de Vries, Joost P. H. Schoeber, Anne J. M. Loonen, Adriaan J. C. van den Brule, Maarten Merkx   +8 more
wiley   +2 more sources

A Myocyte‐Enriched Long Non‐Coding RNA NRMLncR Enhances Myogenesis in Mouse

The FASEB Journal, Volume 40, Issue 13, 15 July 2026.
We identified a novel myocyte‐enriched, Notch‐repressed myogenic lncRNA, NRMLncR, that is induced by myogenic regulatory factors (MRFs) during myogenic progression in mouse. NRMLncR localizes to cytoplasmic and nuclear compartments, associates with the RNA‐binding protein CUGBP Elav‐like family member 1 (CELF1), and is linked to neighboring gene Tbx1 ...
Yufen Li, Yumei Zhou, Qing Ying Li, Justine Arrington, Zhengzheng Hu, Mostafa F. Abdelhai, Yubo Wang, Junxiao Ren, Yung‐Yu Cheng, Mingyi Xie, W. Andy Tao, Shihuan Kuang, Feng Yue   +12 more
wiley   +1 more source

MUTYH Cancer‐Associated Variants Within the Interdomain Connector Differentially Impact Glycosylase Activity and Cellular DNA Repair

ChemBioChem, Volume 27, Issue 13, 14 July 2026.
Functional analysis of cancer‐associated variants of the DNA repair enzyme MUTYH in the interdomain connector between the 8‐oxoguanine (OG) recognition and base excision domains reveals discordance between in vitro assays and OG:A repair in cells. This disconnect highlights the use of complementary biochemical and cellular assays to accurately classify
Cindy Khuu, Melody Malek, Savannah G. Conlon, Geoffrey P. Wadey, Carlos H. Trasviña‐Arenas, Sheila S. David   +5 more
wiley   +1 more source

Design and Synthesis of Novel Imidazo[4,5‐c]pyridine Derivatives, Evaluation of Their Activity Against Hepatitis C Virus and In Silico Prediction of Their Binding Mode to NS4B Protein

ChemMedChem, Volume 21, Issue 13, 14 July 2026.
A series of 18 novel imidazo[4,5‐c]pyridines was synthesized, and their anti‐HCV activity was evaluated. Compound 30b was identified as the most promising one, with EC50 = 6.61 µM. Molecular docking studies indicated the NS4B protein of the HCV as a potential target of these derivatives.
Eftychia Karampella, George Mpekoulis, Maria Georgiou, Iris Kolida, Katerina I. Kalliampakou, Eirini P. Kollia, Eleni V. Mikropoulou, Maria Halabalaki, Christos T. Chasapis, Niki Vassilaki, Nikolaos Lougiakis   +10 more
wiley   +1 more source

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Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen mRNA

Clinical Biochemistry, 1997
To develop a quantitative reverse transcriptase-polymerase chain reaction assay for monitoring the prostate-specific antigen (PSA) mRNA.PSA mRNA is amplified, in parallel, with the mRNA of beta-actin, a housekeeping gene. The ratio of the amplification products obtained reflects the relative amount of PSA mRNA with respect to actin mRNA.
Theodore Christopoulos, T K Christopoulos   +1 more
exaly   +3 more sources

Detection of flaviviruses by reverse‐transcriptase polymerase chain reaction

Journal of Medical Virology, 1991
AbstractRNA sequences of five flaviviruses were detected by a modified polymerase chain reaction (PCR) that incorporated a reverse transcriptase and RNase inhibitor. Oligonucleotide primer pairs were synthesized to amplify sequences from St. Louis encephalitis (SLE), Japanese encephalitis (JBE), yellow fever (YF), dengue 2 (DEN‐2), and dengue 4 (DEN‐4)
Z A, Eldadah, D M, Asher, M S, Godec, K L, Pomeroy, L G, Goldfarb, S M, Feinstone, H, Levitan, C J, Gibbs, D C, Gajdusek   +8 more
openaire   +2 more sources

Nested Reverse Transcriptase‐Polymerase Chain Reaction for the Detection of Group A Rotaviruses

Journal of Veterinary Medicine, Series B, 2002
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine,
M, Elschner, J, Prudlo, H, Hotzel, P, Otto, K, Sachse   +4 more
openaire   +2 more sources

Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus

Avian Diseases, 2005
A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian encephalomyelitis virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural protein P1 region of the AEV genome.
Zhiqin, Xie, Mazhar I, Khan, Theodore, Girshick, Zhixun, Xie   +3 more
openaire   +2 more sources

Reverse Transcriptase In Situ Polymerase Chain Reaction in Atypical Mycobacterial Adenitis

Archives of Otolaryngology - Head and Neck Surgery, 1996
To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis.Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis.University medical center caring for both ambulatory and hospitalized
M M, April, J M, Garelick, G J, Nuovo
openaire   +2 more sources