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Underperformance of Reverse-Transcriptase Polymerase Chain Reaction in Japan and Potential Implications From Diamond Princess Cruise Ship and Other Countries During the Ongoing COVID-19 Pandemic. [PDF]

Int J Health Policy Manag, 2020
Yamamoto K, Ozaki A, Senoo Y, Sawano T, Tanimoto T, Sah R, Wang J.   +6 more
europepmc   +1 more source

Recurrent Positive Reverse Transcriptase-Polymerase Chain Reaction Results for Coronavirus Disease 2019 in Patients Discharged From a Hospital in China.

JAMA Netw Open, 2020
Hu R, Jiang Z, Gao H, Huang D, Jiang D, Chen F, Li J.   +6 more
europepmc   +1 more source

A Reverse Transcriptase Polymerase Chain Reaction Assay for the Detection of thermophilicCampylobacterspp.

Molecular and Cellular Probes, 1998
A novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR). The RNA extraction method, DNase treatment and RT-PCR assay were shown to be specific for mRNA.
D R A Wareing, A J Fox, A J Fox
exaly   +3 more sources

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Detection of flaviviruses by reverse‐transcriptase polymerase chain reaction

Journal of Medical Virology, 1991
AbstractRNA sequences of five flaviviruses were detected by a modified polymerase chain reaction (PCR) that incorporated a reverse transcriptase and RNase inhibitor. Oligonucleotide primer pairs were synthesized to amplify sequences from St. Louis encephalitis (SLE), Japanese encephalitis (JBE), yellow fever (YF), dengue 2 (DEN‐2), and dengue 4 (DEN‐4)
Z A, Eldadah, D M, Asher, M S, Godec, K L, Pomeroy, L G, Goldfarb, S M, Feinstone, H, Levitan, C J, Gibbs, D C, Gajdusek   +8 more
openaire   +2 more sources

Application of Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Urological Oncology

Journal of Urology, 2003
During the last decade numerous different reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been described. However, the lack of highly sensitive, quantitative and reliable methodology has been responsible for its limited use in modern urology.
Axel Heidenreich, Jan Buer, Rainer Hofmann   +2 more
exaly   +3 more sources

Nested Reverse Transcriptase‐Polymerase Chain Reaction for the Detection of Group A Rotaviruses

Journal of Veterinary Medicine, Series B, 2002
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine,
M, Elschner, J, Prudlo, H, Hotzel, P, Otto, K, Sachse   +4 more
openaire   +2 more sources

Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus

Avian Diseases, 2005
A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian encephalomyelitis virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural protein P1 region of the AEV genome.
Zhiqin, Xie, Mazhar I, Khan, Theodore, Girshick, Zhixun, Xie   +3 more
openaire   +2 more sources

Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction

Molecular and Biochemical Parasitology, 2000
A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript ...
M, Menegon, C, Severini, A, Sannella, M G, Paglia, D, Sangaré, A, Abdel-Wahab, A A, Abdel-Muhsin, H, Babiker, D, Walliker, P, Alano   +9 more
openaire   +2 more sources

Detection of measles virus by reverse-transcriptase polymerase chain reaction in a placenta

The Journal of Maternal-Fetal & Neonatal Medicine, 2009
Measles virus (MV) during pregnancy is associated with maternal morbidity and mortality and can put the fetus and newborn at risk of a wide range of complications. Reverse-transcriptase polymerase chain reaction (RT-PCR) for detecting MV in the placenta has not been reported.A case of RT-PCR detection of MV in the placenta of a 38-year-old woman who ...
Shikma, Bar-On, Yifat, Ochshorn, Ora, Halutz, Yair, Aboudy, Ariel, Many   +4 more
openaire   +2 more sources

Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Hongxin, Fan, Ryan S, Robetorye
openaire   +2 more sources