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ABSTRACT Background Skin aging is driven by intrinsic and extrinsic factors, with ultraviolet (UV) radiation recognized as the primary cause of photoaging. Photoaged skin is characterized by reduced dermal thickness and collagen content. Recently, a liquid form of polycaprolactone (PCL) designed to promote uniform scaffold formation within the skin has
Jimyung Seo +9 more
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Summary Plant resistance influences the host range of pathogens; however, the mechanism driving this influence remains poorly understood. Brassicaceae species are not natural hosts of potexviruses, for reasons that have not yet been determined. This study investigated the molecular basis of nonhost resistance (NHR) to potexviruses in the Brassicaceae ...
Takumi Suzuki +11 more
wiley +1 more source
Cytokinin–phyB signaling modulates PSII photochemistry during prolonged darkness. Summary Cytokinins (CKs) delay dark‐induced senescence, but how they tune photosynthetic function in darkness remains unclear. We investigated the effects of classical aromatic CK benzylaminopurine and CK‐derived compound 1‐(2‐methoxyethyl)‐3‐(1,2,3‐thiadiazol‐5‐yl)urea ...
Veronika Kábrtová +14 more
wiley +1 more source
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Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen mRNA
Clinical Biochemistry, 1997To develop a quantitative reverse transcriptase-polymerase chain reaction assay for monitoring the prostate-specific antigen (PSA) mRNA.PSA mRNA is amplified, in parallel, with the mRNA of beta-actin, a housekeeping gene. The ratio of the amplification products obtained reflects the relative amount of PSA mRNA with respect to actin mRNA.
Theodore K Christopoulos
exaly +3 more sources
Detection of flaviviruses by reverse‐transcriptase polymerase chain reaction
Journal of Medical Virology, 1991AbstractRNA sequences of five flaviviruses were detected by a modified polymerase chain reaction (PCR) that incorporated a reverse transcriptase and RNase inhibitor. Oligonucleotide primer pairs were synthesized to amplify sequences from St. Louis encephalitis (SLE), Japanese encephalitis (JBE), yellow fever (YF), dengue 2 (DEN‐2), and dengue 4 (DEN‐4)
Z A, Eldadah +8 more
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Nested Reverse Transcriptase‐Polymerase Chain Reaction for the Detection of Group A Rotaviruses
Journal of Veterinary Medicine, Series B, 2002Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine,
M, Elschner +4 more
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Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus
Avian Diseases, 2005A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian encephalomyelitis virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural protein P1 region of the AEV genome.
Zhiqin, Xie +3 more
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Reverse Transcriptase In Situ Polymerase Chain Reaction in Atypical Mycobacterial Adenitis
Archives of Otolaryngology - Head and Neck Surgery, 1996To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis.Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis.University medical center caring for both ambulatory and hospitalized
M M, April, J M, Garelick, G J, Nuovo
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