Results 231 to 240 of about 4,181,603 (269)

Reverse Transcriptase In Situ Polymerase Chain Reaction in Atypical Mycobacterial Adenitis

Archives of Otolaryngology - Head and Neck Surgery, 1996
To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis.Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis.University medical center caring for both ambulatory and hospitalized
M M, April, J M, Garelick, G J, Nuovo
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Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

2003
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene ...
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Detection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction

2003
In oncology, the correct assessment of tumor stage is crucial information, as it relates to disease-free interval and prognosis. Routine staging modalities use histopathology to evaluate the presence or absence of metastasis mainly in tissues such as lymph nodes, as described in Chapter 1 by Roskell and Buley.
K, Haack, K, Soondrum, M, Neumaier
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A Reverse Transcriptase-Polymerase Chain Reaction Assay for the Detection and Quantitation of Murine Retroviruses

Nature Biotechnology, 1993
Specific hybridization primers for the PCR assay were developed to detect the presence of the ecotropic, xenotropic, and mink cell focus-forming classes of murine leukemia viruses (MuLVs) in samples derived from cultured cells and cell-free supernatants.
J M, Irving, L W, Chang, F J, Castillo
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Detection of Rotaviruses in the Day Care Environment by Reverse Transcriptase Polymerase Chain Reaction

Journal of Infectious Diseases, 1992
Group A rotavirus is an important cause of morbidity among infants and toddlers in day care centers. Transmission by the fecal-oral route is well established, but fomites and environmental surfaces may also play an important role in transmission. A highly sensitive polymerase chain reaction (PCR) assay was used to detect rotavirus RNA in day care ...
J, Wilde, R, Van, L, Pickering, J, Eiden, R, Yolken   +4 more
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Detection and Identification of Flaviviruses by Reverse Transcriptase Polymerase Chain Reaction

1992
We developed a reverse transcriptase/polymerase chain reaction (RT/PCR) for the detection and identification of flavivirus RNA in a single-tube assay. Universal flavivirus RT/PCR amplimers amplify an 832-bp region in the carboxyl portion of NS5 (nt9166-9997) which contains nucleotide sequences conserved among all flaviviruses at the 3′- and 5 ...
Dennis W. Trent, Gwong-Jen Chang
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A Reverse Transcriptase-Polymerase Chain Reaction Assay for Detecting Highlands J Virus

Avian Diseases, 2001
Highlands J (HJ) virus is an arbovirus frequently recovered at high rates in mosquitoes collected in the eastern United States. HJ virus is primarily a veterinary pathogen causing disease in domestic birds including turkeys, chickens, and partridges.
Whitehouse, C. A., Guibeau, A., McGuire, D., Takeda, T., Mather, T. N.   +4 more
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Quantification of poliovirus in seawater and sewage by competitive reverse transcriptase - polymerase chain reaction

Canadian Journal of Microbiology, 1998
Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time. However, unlike traditional cell culture, RT-PCR has not provided quantitation and infectivity information.
Y L, Tsai, S L, Parker
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Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen mRNA

Clinical Biochemistry, 1997
To develop a quantitative reverse transcriptase-polymerase chain reaction assay for monitoring the prostate-specific antigen (PSA) mRNA.PSA mRNA is amplified, in parallel, with the mRNA of beta-actin, a housekeeping gene. The ratio of the amplification products obtained reflects the relative amount of PSA mRNA with respect to actin mRNA.
B, Galvan, T K, Christopoulos
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Early diagnosis of murray valley encephalitis by reverse transcriptase-polymerase chain reaction

Pathology, 2000
A 4-year-old aboriginal boy developed encephalitis due to Murray Valley encephalitis virus (MVE) following an earlier infection with Kunjin virus (KUN). The illness was severe, resulting in cerebral atrophy and profound physical and intellectual disability.
P C, McMinn, P G, Carman, D W, Smith
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