Results 41 to 50 of about 515,505 (407)

Direct in situ reverse transcriptase-polymerase chain reaction [PDF]

American Journal of Physiology-Cell Physiology, 2001
In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
Robert L. Bacallao, Rajesh Kher
openaire   +3 more sources

Reverse Transcriptase-Polymerase Chain Reaction

Definitions, 2020
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA strand into a DNA complement is termed reverse transcription and is done by the enzyme reverse ...

semanticscholar   +1 more source

Accuracy of Rapid Antigen Detection Test Device (COVID-19 Ag Rapid Test Device) in Diagnosis of Acute COVID-19 Infections

Pakistan Armed Forces Medical Journal, 2022
Objective: To evaluate the accuracy of the COVID-19 Ag Rapid test device for detection of SARS-CoV-2 with Reverse Transcriptase Polymerase Chain Reaction as the gold standard. Study Design: Cross-sectional study.
Noman Shakoor, Eijaz Ghani, Saifullah Khan Niazi, Misbah Noor, Faraz Ahmed, Hammad Hussain   +5 more
doaj   +1 more source

REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAYS FOR PROSTATE CANCER [PDF]

Urologic Clinics of North America, 1997
RT-PCR, if targeted against a prostate-specific marker, can be a highly specific and sensitive assay to detect the presence of occult prostate cancer cells at sites far distant from the primary tumor. The low false-positive rate (0.8%) observed when combining most published studies and the high rate of detection of prostate cells in metastatic patients
Glen M. de Vries, Aaron E. Katz, Ralph Buttyan, Carl A. Olsson   +3 more
openaire   +2 more sources

False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Korean Journal of Radiology, 2020
The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide.
Dasheng Li, Dawei Wang, Jianping Dong, Nan Wang, He Huang, Haiwang Xu, Chen Xia   +6 more
semanticscholar   +1 more source

Long term persistence of coronavirus SARS-CoV-2 infection

Einstein (São Paulo), 2021
During the COVID-19 pandemic, a case of a long-term persistence of SARS-CoV-2 infection (from March 26 to May 20, 2020) was identified at a private hospital in São Paulo, SP, Brazil.
João Renato Rebello Pinho, Ketti Gleyzer de Oliveira, Roberta Sitnik, Maira Marranghello Maluf, Pedro Henrique Sebe Rodrigues, Rúbia Anita Ferraz Santana, Eliane Rosseto Welter, Ophir Irony   +7 more
doaj   +2 more sources

Acute viral gastroenteritis in children hospitalized in Iksan, Korea during December 2010-June 2011 [PDF]

Korean Journal of Pediatrics, 2013
PurposeViral etiology is common in cases of children with acute diarrhea, and antibiotic therapy is usually not required. Therefore, it is important to determine the distribution of common viruses among children hospitalized with acute diarrhea.MethodsWe
Cheol Whoan So, Dong Sup Kim, Seung Taek Yu, Ji-Hyun Cho, Jong Duck Kim   +4 more
doaj   +1 more source

Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction. [PDF]

Archives of Disease in Childhood, 1996
The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
J Hodgson, J.C. Booth, S Jeffery, Mike Sharland, E G Davies   +4 more
openaire   +3 more sources

Automated diagnosis of COVID-19 with limited posteroanterior chest X-ray images using fine-tuned deep neural networks [PDF]

Appl Intell (2020), 2020
The novel coronavirus 2019 (COVID-19) is a respiratory syndrome that resembles pneumonia. The current diagnostic procedure of COVID-19 follows reverse-transcriptase polymerase chain reaction (RT-PCR) based approach which however is less sensitive to identify the virus at the initial stage.
arxiv   +1 more source

Utilization of a deoxynucleoside diphosphate substrate by HIV reverse transcriptase [PDF]

, 2008
Background: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA sysnthesis is catalyzed by polymerases such as HIV-1 reverse transcriptase (RT).
A Bevington, A Mas, AJ Smith, B Selmi, BA Barshop, BA Mulder, BR Bochner, C Cruchaga, C Isnard Bagnis, CK Tan, D Arion, EF Pettersen, G Pontarin, G Pontarin, G Yang, G Yang, GJ Kemp, H Huang, H Ling, H Pelletier, H Pelletier, HK Schachman, IA Nazarenko, K Bebenek, KL White, Kornberg, Linqi Zhang, M Rigourd, MC Franklin, Michael A. Parniak, MJ Bessman, MK Kukhanova, N Sluis-Cremer, PJ Rothwell, PL Boyer, PM Burgers, PM Burgers, PR Meyer, PR Meyer, PR Meyer, PR Meyer, PR Meyer, RS Fletcher, S Dharmasena, Scott J. Garforth, SJ Garforth, SV Doronin, SV Doronin, TA Kunkel, TA Rozovskaya, TA Steitz, UM Parikh, Vinayaka R. Prasad, Z Gu   +53 more
core   +9 more sources