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Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and its usefulness in soil microbial ecological studies - A review [PDF]
, 2014 The reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a highly specific polymerase chain reaction (PCR) method that allows one to detect very low transcription levels of functional gene(s) in soil.
Asuming-Brempong, S
core +2 more sources
Academic Pathology, 2021 Molecular techniques, especially reverse transcriptase polymerase chain reaction (RT-PCR), have been the gold standard for the diagnosis of acute severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection.
Robert M. Humble MD +4 more
doaj +1 more source
Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]
, 2011 The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Lorraine O'Driscoll, Sweta Rani
openaire +5 more sources
Animal Research and One Health, EarlyView., 2023 In our study, we conducted circRNA expression profiling of Zhongwei goat skin exhibiting divergent curvature wool phenotypes at two developmental stages using the RNA‐seq. Functional verification assay demonstrated that circRNA8782 regulated fibroblast proliferation.
Yingxiao Su +9 more
wiley +1 more source
Molecular diagnosis of peste des petits ruminants virus (PPR) in goats and sheep populations [PDF]
, 2022 Peste des petits ruminants (PPR) is an economically important viral disease of goats and sheep. The disease is confused clinically with other infections such as the mild strain of rinderpest in small ruminants.
Abdel-Rady, A
core +1 more source
What are we missing? Three cases of severe COVID-19 pneumonia with negative testing
SAGE Open Medical Case Reports, 2020 The COVID-19 pandemic has drastically affected health care systems globally. Reverse transcriptase-polymerase chain reaction is currently the preferred method of detecting COVID-19; however, sensitivity of this test remains questionable.
Aisha Siddiqui +5 more
doaj +1 more source
The Iraqi Journal of Agricultural science, 2020 This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the ...
Fadhil & et al.
doaj +1 more source
Stabilized reverse transcriptase fusion proteins [PDF]
, 2016 Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and ...
Alan M. Lambowitz +3 more
core +1 more source
Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]
The Analyst, 2018 We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng +4 more
openaire +6 more sources
The Pan African Medical Journal, 2021 Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede +17 more
doaj +1 more source