Multiplexed Molecular Profiling of Lung Cancer Using Pleural Effusion [PDF]
Introduction:Pleural effusion is frequently observed in patients with advanced lung cancer. Although effusion can be obtained less invasively and repeatedly, its use in multiplexed molecular profiling has not been fully investigated.Methods:Between July ...
Akamatsu, Hiroaki+16 more
core +1 more source
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR).
Meadus W.J.
doaj +1 more source
Capacity of rTth polymerase to detect RNA in the presence of various inhibitors. [PDF]
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical ...
Dongyang Cai+4 more
doaj +1 more source
The polymerase chain reaction-based SARS-CoV-2 detection
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa+2 more
doaj +2 more sources
Fetal Cytokine Expression In UteroDetected by Reverse Transcriptase Polymerase Chain Reaction [PDF]
Cytokine expression at the maternal-fetal interface is well documented. Some authors have postulated the existence of a bidirectional cytokine signaling mechanism that is critical to the maintenance of normal pregnancy. The role of the fetus (versus fetally derived placental tissues) in this process is unknown.
Liangping Deng+4 more
openaire +3 more sources
Early Diagnostic Prediction of Covid-19 using Gradient-Boosting Machine Model [PDF]
With the huge spike in the COVID-19 cases across the globe and reverse transcriptase-polymerase chain reaction (RT-PCR) test remains a key component for rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In recent months there has been an acute shortage of medical supplies in developing countries, especially a
arxiv
Quantitative Reverse Transcriptase Polymerase Chain Reaction
Since the first documentation of real-time polymerase chain reaction (PCR),(1) it has been used for an increasing and diverse number of applications, including mRNA expression studies, DNA copy number measurements in genomic or viral DNAs,(2–7) allelic discrimination assays,(8,9) expression analysis of specific splice variants of genes(10–13) and gene ...
Prabhabhai S. Patel+4 more
openaire +2 more sources
DCNNV-19: A Deep Convolutional Neural Network for COVID-19 Detection in Chest Computed Tomographies [PDF]
This technical report proposes the use of a deep convolutional neural network as a preliminary diagnostic method in the analysis of chest computed tomography images from patients with symptoms of Severe Acute Respiratory Syndrome (SARS) and suspected COVID-19 disease, especially on occasions when the delay of the RT-PCR result and the absence of urgent
arxiv
Computational Investigations on Polymerase Actions in Gene Transcription and Replication Combining Physical Modeling and Atomistic Simulations [PDF]
Polymerases are protein enzymes that move along nucleic acid chains and catalyze template-based polymerization reactions during gene transcription and replication. The polymerases also substantially improve transcription or replication fidelity through the non-equilibrium enzymatic cycles.
arxiv +1 more source
Early changes in rat diaphragm biology with mechanical ventilation [PDF]
To better characterize the effects of 24-hour mechanical ventilation on diaphragm, the expression of myogenic transcription factors, myosin heavy chains, and sarcoplasmic/endoplasmic reticulum calcium-ATPase pumps was examined in rats.
Cadot, Pascal+7 more
core +1 more source