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Expanding the reverse transcription toolbox
Nature Chemistry, 2020Tailor-made reverse transcriptases are used in molecular biology and synthetic genetics. However, re-engineering these enzymes to work with non-natural nucleic acids is difficult and requires powerful directed evolution strategies. Now, an adaptable selection approach has been demonstrated for the evolution of new reverse transcriptases.
Melanie Henkel, Andreas Marx
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Reverse-engineering transcription control networks
Physics of Life Reviews, 2005Microarray technologies, which enable the simultaneous measurement of all RNA transcripts in a cell, have spawned the development of algorithms for reverse-engineering transcription control networks. In this article, we classify the algorithms into two general strategies: physical modeling and influence modeling.
Timothy S, Gardner, Jeremiah J, Faith
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APOBEC3 proteins and reverse transcription
Virus Research, 2008The ability of members of the APOBEC3 (A3) family of proteins to confer intrinsic immunity to retroviral infection was recognized in several studies. More specifically, A3 proteins are cytidine deaminases (CDAs) that cause hypermutations of nascent retroviral genomes by deamination of cytidine residues. Although A3 proteins can restrict the replication
Renato S, Aguiar, B Matija, Peterlin
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Retroviral Recombination and Reverse Transcription
Science, 1990Recombination occurs at a high rate in retroviral replication, and its observation requires a virion containing two different RNA molecules (heterodimeric particles). Analysis of retroviral recombinants formed after a single round of replication revealed that (i) the nonselected markers changed more frequently than expected from the rate of ...
W S, Hu, H M, Temin
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Trends in Biochemical Sciences, 1985
Abstract Cauliflower mosaic virus, although a DNA virus, uses RNA as a replicative intermediate and employs reverse transcriptase for genome replication. Details of this replication mode as currently known and implications for other aspects of the life cycle of this virus are presented here.
T. Hohn, B. Hohn, P. Pfeiffer
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Abstract Cauliflower mosaic virus, although a DNA virus, uses RNA as a replicative intermediate and employs reverse transcriptase for genome replication. Details of this replication mode as currently known and implications for other aspects of the life cycle of this virus are presented here.
T. Hohn, B. Hohn, P. Pfeiffer
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Reverse transcription in genome evolution
Cytogenetic and Genome Research, 2005As reverse transcription is predominantly performed by retrotransposable elements, the process is often entirely associated with the propagation of these elements. However, as a unique tool for transmitting information from the dynamic RNA to the more inert DNA, reverse transcription has been instrumental in shaping extant genomes.
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Reverse Transcription–Polymerase Chain Reaction
Cold Spring Harbor Protocols, 2014Reverse transcription coupled to the polymerase chain reaction (RT–PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest. For mRNA, the primer is usually a synthetic oligo(dT)15–18, a random hexamer mixture (dN)6, or a synthetic ...
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Reverse Engineering Transcriptional Gene Networks
2013The aim of this chapter is a step-by-step guide on how to infer gene networks from gene expression profiles. The definition of a gene network is given in Subheading 1, where the different types of networks are discussed. The chapter then guides the readers through a data-gathering process in order to build a compendium of gene expression profiles from ...
Belcastro V, DI BERNARDO, DIEGO
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Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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