Results 161 to 170 of about 1,031,121 (258)

Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry. [PDF]

Pathogens
Yehia N, Abd El Wahed A, Mohamed AAE, Arafa A, Said D, Shalaby MA, Ceruti A, Truyen U, Kobialka RM.   +8 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Rapid and Visual Detection of Muscovy Duck Reovirus Using a Reverse Transcription Recombinase-Aided Amplification Assay in a Lyophilized Format for Point-of-Care Applications. [PDF]

Transbound Emerg Dis
Chen X, Zhang S, Luo X, You G, Chen S, Huang M, Wang S, Chen S.   +7 more
europepmc   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

A robust absolute quantification protocol for potato virus Y based on reverse transcription-quantitative PCR. [PDF]

MicroPubl Biol
Spencer D, Padilla-Crespo E, Tan EH.
europepmc   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

A CRISPR/Cas12a-based reverse transcription multiple cross displacement amplification technique for sensitive, rapid, and highly specific detection of <i>human enterovirus A71</i> in clinical application. [PDF]

Front Microbiol
Zhang X, Ye R, Liang Q, Cao Y, Liu M, Wei F, Zhu Y, Yuan Y, Guo G, Wang Y.   +9 more
europepmc   +1 more source

Reverse Transcription Inhibition [PDF]

, 2020
openaire   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria. [PDF]

Appl Environ Microbiol
Hane M, Shimamoto K, Matsui R, Shimizu K, Katto M, Asahara T, Shishido Y, Kurakawa T.   +7 more
europepmc   +1 more source