Development of a Conventional Reverse Transcription-PCR System for Broad Detection of Bovine Rhinitis A Virus. [PDF]
Koizumi S, Nishi T, Morioka K, Fukai K.
europepmc +1 more source
Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde +3 more
wiley +1 more source
Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry. [PDF]
Yehia N +8 more
europepmc +1 more source
PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart +7 more
wiley +1 more source
A robust absolute quantification protocol for potato virus Y based on reverse transcription-quantitative PCR. [PDF]
Spencer D, Padilla-Crespo E, Tan EH.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria. [PDF]
Hane M +7 more
europepmc +1 more source

